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主营:主营:研究并生产荧光和发光探针,信号转导研究的试剂
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当前位置: 首页 > 产品中心 > Fluorescent_dyes > AAT Bioquest/6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] *CAS#: 877049-90-6*/6027/100 µmoles
商品详细AAT Bioquest/6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] *CAS#: 877049-90-6*/6027/100 µmoles
AAT Bioquest/6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] *CAS#: 877049-90-6*/6027/100 µmoles
AAT Bioquest/6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] *CAS#: 877049-90-6*/6027/100 µmoles
商品编号: 6027
品牌: aatbio
市场价: ¥3300.00
美元价: 1980.00
产地: 美国(厂家直采)
公司:
产品分类: 荧光染料
公司分类: Fluorescent_dyes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Overview
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Ex/Em(nm)521/541
MW981.72
CAS#877049-90-6
SolventMeCN
StorageF/D/L
CategoryOligoSynthesis
DyePhosphoramidites
RelatedHydroxy-ReactiveProbes
BiochemicalAssays
Thereareseveralwaysoflabelinganoligonucleotidewithfluorescein.Thechoiceoflabelisdiversifiedfurther,dependingonthespectralrequirements.5"-fluorescein-CEphosphoramidite,derivedfromthesingleisomer6-carboxyfluorescein,5"-hexachlorofluorescein-CEphosphoramidite(HEX)and5"-tetrachlorofluorescein-CEphosphoramidite(TET)canallbeusedtoefficientlylabelanoligonucleotideatthe5"-end.Labelingthe3"-endofanoligowithfluoresceincanbeachievedusing3"-FluoresceinCPGs.Standardcleavageanddeprotectionwithammoniumhydroxideliberatesthefluorescein-labeledoligowhenusinganyofthesesupports.
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
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References&Citations
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1.   BrowneKA.(2005)Sequence-specific,self-reportinghairpininversionprobes.JAmChemSoc,127,1989.

2.   DobsonN,McDowellDG,FrenchDJ,BrownLJ,MellorJM,BrownT.(2003)SynthesisofHyBeaconsanddual-labelledprobescontaining2"-fluorescentgroupsforuseingeneticanalysis.ChemCommun(Camb),1234.

3.   HagmarP,BaileyM,TongG,HaralambidisJ,SawyerWH,DavidsonBE.(1995)Synthesisandcharacterisationoffluorescentoligonucleotides.Effectofinternallabellingonproteinrecognition.BiochimBiophysActa,1244,259.

4.   HakalaH,VirtaP,SaloH,LonnbergH.(1998)Simultaneousdetectionofseveraloligonucleotidesbytime-resolvedfluorometry:theuseofamixtureofcategorizedmicroparticlesinasandwichtypemixed-phasehybridizationassay.NucleicAcidsRes,26,5581.

5.   HillKW,Taunton-RigbyJ,CarterJD,KroppE,VagleK,PiekenW,McGeeDP,HusarGM,LeuckM,AnzianoDJ,SebestaDP.(2001)Diels--Alderbioconjugationofdiene-modifiedoligonucleotides.JOrgChem,66,5352.

6.   KimSJ,BangEK,KwonHJ,ShimJS,KimBH.(2004)Modifiedoligonucleotidescontaininglithocholicacidintheirbackbones:theirenhancedcellularuptakeandtheirmimickingofhairpinstructures.Chembiochem,5,1517.

7.   KutyavinIV,LokhovSG,AfoninaIA,DempcyR,GallAA,GornVV,LukhtanovE,MetcalfM,MillsA,ReedMW,SandersS,ShishkinaI,VermeulenNM.(2002)ReducedaggregationandimprovedspecificityofG-richoligodeoxyribonucleotidescontainingpyrazolo[3,4-d]pyrimidineguaninebases.NucleicAcidsRes,30,4952.

8.   LeeSP,CensulloML,KimHG,KnutsonJR,HanMK.(1995)CharacterizationofendonucleolyticactivityofHIV-1integraseusingafluorogenicsubstrate.AnalBiochem,227,295.

9.   MeyerKL,HannaMM.(1996)Synthesisandcharacterizationofanew5-thiol-protecteddeoxyuridinephosphoramiditeforsite-specificmodificationofDNA.BioconjugChem,7,401.

10.   NelsonPS,KentM,MuthiniS.(1992)Oligonucleotidelabelingmethods.3.Directlabelingofoligonucleotidesemployinganovel,non-nucleosidic,2-aminobutyl-1,3-propanediolbackbone.NucleicAcidsRes,20,6253.


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品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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