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主营:主营:研究并生产荧光和发光探针,信号转导研究的试剂
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当前位置: 首页 > 产品中心 > Labeling_Kit > AAT Bioquest/ReadiLink™快速Cy5抗体标记试剂盒*微型优化,用于标记每个反应50µg抗体
商品详细AAT Bioquest/ReadiLink™快速Cy5抗体标记试剂盒*微型优化,用于标记每个反应50µg抗体
AAT Bioquest/ReadiLink™快速Cy5抗体标记试剂盒*微型优化,用于标记每个反应50µg抗体
AAT Bioquest/ReadiLink™快速Cy5抗体标记试剂盒*微型优化,用于标记每个反应50µg抗体
商品编号: 1292
品牌: aatbio
市场价: ¥42060.00
美元价: 25236.00
产地: 美国(厂家直采)
公司:
产品分类: 标记试剂盒
公司分类: Labeling_Kit
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
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Ex/Em(nm)649/665
MWN/A
CAS#N/A
SolventDMSO
StorageF/D/L
CategorySuperiorLabelingDyes
iFluor™DyesandKits
RelatedGeneralproteins
LabelingviaAminoGroups
Cy5isoneofthemostpopularfluorescentlabelingdyesforpreparingorange-redfluorescentbioconjugates.However,mostofthecommercialCy5labelingkitsrequireintensivehands-ontime.ThisCy5ReadiLink™labelingkitisoneofthemostrobustproteinlabelingkitsforpreparingCy5-labeledantibodyconjugatesorotherproteinconjugates.Itessentiallyonlyrequires2simplemixingstepswithoutacolumnpurificationrequired.Thekitprovidesalltheessentialcomponentsforlabeling~2x50ugantibody.EachofthetwovialsofCy5dyeprovidedinthekitisoptimizedforlabeling~50µgantibody.ThisCy5proteinlabelingkitprovidesaconvenientmethodtolabelmonoclonal,polyclonalantibodiesorotherproteins(>10kDa).
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
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Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.
  1. Prepareproteinsolution(SolutionA):
    Forlabeling50ugprotein(assumingthetargetproteinconcentrationis1mg/mL),mix5μL(10%ofthetotalreactionvolume)ofReactionBuffer(ComponentB)with50uLofthetargetproteinsolution.
    Note1:Ifyouhaveadifferenceproteinconcentration,adjusttheproteinvolumeaccordinglytomake~50µgproteinavailableforyourlabelingreaction.
    Note2:Forlabeling100ugprotein(assumingthetargetproteinconcentrationis1mg/mL),mix10uL(10%ofthetotalreactionvolume)ofReactionBuffer(ComponentB)with100uLofthetargetproteinsolution.
    Note3:Theproteinshouldbedissolvedin1Xphosphatebufferedsaline(PBS),pH7.2-7.4;Iftheproteinisdissolvedinglycinebuffer,itmustbedialyzedagainst1XPBS,pH7.2-7.4,oruseAmiconUltra-0.5,Ultracel-10Membrane,10kDa(cat#UFC501008fromMillipore)toremovefreeaminesorammoniumsalts(suchasammoniumsulfateandammoniumacetate)thatarewidelyusedforproteinprecipitation.
    Note4:ImpureantibodiesorantibodiesstABIlizedwithbovineserumalbumin(BSA)orgelatinwillnotbelabeledwell.
    Note5:Theconjugationefficiencyissignificantlyreducediftheproteinconcentrationislessthan1mg/mL.Foroptimallabelingefficiencythefinalproteinconcentrationrangeof1-2mg/mLisrecommended.

  2. Runconjugationreaction::
    1. Addtheproteinsolution(SolutionA)toONEvialoflabelingdye(ComponentA),andmixthemwellbyrepeatedlypipettingforafewtimesorvortexthevialforafewseconds.
      Note:Usebothvials(ComponentA)oflabelingdyetolabel100ugproteinbydividingthe100ugproteininto2x50ugproteinandreactingeach50ugproteinwithonevialoflabelingdye.Combinetwovialsforthenextstep.
    2. Keeptheconjugationreactionmixtureatroomtemperaturefor30-60minutes.
      Note:Theconjugationreactionmixturecanberotatedorshakenforlongertimeifdesired.

  3. StopConjugationreaction:
    1. Add5uL(for50ugprotein)or10uL(for100ugprotein)whichis10%ofthetotalreactionvolumeofTQ-DyedQuenchBuffer(ComponentC)intotheconjugationreactionmixture(fromstep2.2),mixthemwell.
    2. Incubateatroomtemperaturefor10minutes.
    3. Thelabeledprotein(antibody)isnowreadytouse.
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品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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