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当前位置: 首页 > 产品中心 > Other_kits > AAT Bioquest/Cell Meter™ Fluorimetric Intracellular Nitric oxide (NO) Activity Assay Kit *Red Fluorescence Optimized f
商品详细AAT Bioquest/Cell Meter™ Fluorimetric Intracellular Nitric oxide (NO) Activity Assay Kit *Red Fluorescence Optimized f
AAT Bioquest/Cell Meter™ Fluorimetric Intracellular Nitric oxide (NO) Activity Assay Kit *Red Fluorescence Optimized f
AAT Bioquest/Cell Meter™ Fluorimetric Intracellular Nitric oxide (NO) Activity Assay Kit *Red Fluorescence Optimized f
商品编号: 16356
品牌: aatbio
市场价: ¥100060.00
美元价: 60036.00
产地: 美国(厂家直采)
公司:
产品分类: 其它检测试剂盒
公司分类: Other_kits
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
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Ex/Em(nm)630/660
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategoryNeuroBIOLOGy
ReactiveOxygenSpecies
RelatedCellSignaling
BiochemicalAssays
Nitricoxide(NO)isanimportantbiologicalregulatorinvolvedinnumbersofphysiologicalandpathologicalprocesses.AlteredNOproductionisimplicatedinvariousimmunological,cardiovascular,neurodegenerativeandinflammatorydiseases.AsafreerADIcal,NOisrapidlyoxidizedandthereisrelativelylowconcentrationsofNOexistinginvivo.IthasbeenchallengingtodetectandunderstandtheroleofNOinbiologicalsystems.CellMeter™FluorimetricIntracellularNitricOxideAssayKitprovidesasensitivetooltomonitorintracellularNOlevelinlivecells.Nitrixyte™probesaredevelopedandusedinourkitasanexcellentreplacementforDAF-2forthedetectionandimagingoffreeNOincells.ComparedtothecommonlyusedDAF-2probe,Nitrixyte™probeshavebetterphotostABIlityandenhancedcellpermeability.ThisparticularkitusesNitrixyte™RedthatcanreactwithNOtogenerateabrightredfluorescentproductthathasspectralpropertiessimilartoTexasRed®.Nitrixyte™Redcanbereadilyloadedintolivecells,anditsfluorescencesignalcanbeconvenientlymonitoredusingthefiltersetofRed.Thiskitisoptimizedforflowcytometryapplications.
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
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Movemouseovergridtodisplaywavelength&intensityvalues.

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Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.      Foreachsample,preparecellsin0.5mLwarmmediumorbufferofyourchoiceatadensityof5×105to1×106cells/mL.

Note:EachcelllineshouldbeevaluatedonanindividualbasistodeterminetheoptimalcelldensityforNOinduction.

 

2.       Add1μLof500XNitrixyte™Red(ComponentA)into0.5mLcellsUSPension. 

Note:Foradherentcells,gentlyliftthecellswith0.5mMEDTAtokeepthecellsintact,andwashthecellsoncewithserum-containingmediapriortoincubationwithNitrixyte™Red.

3.      IncubatecellswithtestcompoundsandNitrixyte™Red(fromStep2)at37ºCforadesiredperiodoftimetogenerateendogenousorexogenousNO.

Note1:Theappropriateincubationtimedependsontheindividualcelltypeandtestcompoundused.Optimizetheincubationtimeforeachexperiment.

Note2:WehaveusedRaw264.7cellsincubatedwithNitrixyte™Redworkingsolution,20µg/mLoflipopolysaccharide(LPS)and1mML-Arginine(L-Arg)incellculturemediumat37ºCfor16hours.

 

4.      ForaNONOatepositivecontroltreatment,add200uLofddH2OintothevialofComponentBtomake50mMstocksolution.Thestocksolutionisdilutedtomake1-2mMworkingsolutionwithAssayBuffer(ComponentC).Spindowncellsthathavepre-incubatedwithNitrixyte™Orangefor30minutes.Resuspendcellswith1mMDEANONOatepositivecontrolworkingsolution,andincubateat37ºCforanother30minutes.SeeFigure1fordetails.

 

5.      MonitorthefluorescenceintensityattheFL4channel(Ex/Em=630/660nm)usingaflowcytometer.Gateonthecellsofinterest,excludingdebris.

References&Citations
CitationExplorer

Fluorescentreal-timequantitativemeasurementsofintracellularperoxynitritegenerationandinhibition
Authors:ZhenLuo,QinZhao,JixiangLiu,JinfangLiao,RuoguPeng,YuntingXi,ZhenjunDiwu
Journal:AnalyticalBiochemistry(2017)

InducibleNitricOxideSynthase(iNOS)IsaNovelNegativeRegulatorofHematopoieticStem/ProgenitorCellTrafficking
Authors:MateuszAdamiak,AhmedABDelbaset-Ismail,JosephBMoore,JZhao,AhmedAbdel-Latif,MarcinWysoczynski,MariuszZRatajczak
Journal:StemCellReviewsandReports(2016):1--12


品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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