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主营:主营:研究并生产荧光和发光探针,信号转导研究的试剂
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当前位置: 首页 > 产品中心 > Other_kits > AAT Bioquest/细胞仪与贸易;荧光法细胞内总活性氧活性测定试剂盒*深红色荧光*/22903/200试验
商品详细AAT Bioquest/细胞仪与贸易;荧光法细胞内总活性氧活性测定试剂盒*深红色荧光*/22903/200试验
AAT Bioquest/细胞仪与贸易;荧光法细胞内总活性氧活性测定试剂盒*深红色荧光*/22903/200试验
AAT Bioquest/细胞仪与贸易;荧光法细胞内总活性氧活性测定试剂盒*深红色荧光*/22903/200试验
商品编号: 22903
品牌: aatbio
市场价: ¥56560.00
美元价: 33936.00
产地: 美国(厂家直采)
公司:
产品分类: 其它检测试剂盒
公司分类: Other_kits
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
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Ex/Em(nm)658/675
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategoryNeuroBIOLOGy
ReactiveOxygenSpecies
RelatedCellSignaling
CellFunctionalAnalysis
BiochemicalAssays
Reactiveoxygenspecies(ROS)arenaturalbyproductsofthenormalmetabolismofoxygenandplayimportantrolesincellsignaling.TheaccumulationofROSresultsinsignificantdamagetocellstructures.Theroleofoxidativestressincardiovasculardisease,diabetes,osteoporosis,stroke,inflammatorydiseases,anumberofneurodegenerativediseasesandcancerhasbeenwellestablished.TheROSmeasurementwillhelptodeterminehowoxidativestressmodulatesvariedintracellularpathways.CellMeter™FluorimetricIntracellularTotalROSActivityAssayKitusesourproprietaryROSBrite™670sensortoquantifyROSinlivecells.Thecell-permeableandnon-fluorescentROSBrite™670exhibitsastrongfluorescencesignaluponreactionwithROS.ROSBrite™670sensorislocalizedinthecytoplasm.ThefluorescencesignalofROSBrite™670sensorcanbemeasuredbyfluorescencemicroscopy,high-contentimaging,microplatefluorometry,orflowcytometry.TheCellMeter™FluorimetricIntracellularTotalROSActivityAssayKitprovidesasensitive,one-stepfluorimetricassaytodetectintracellularROS(especiallysuperoxideandhydroxylrADIcal)inlivecellswithin1hourincubation.Theassaycanbeperformedinaconvenient96-wellor384-wellmicrotiter-plateformatusingeitherafluorescencemicroplatereaderatEx/Em=640/670nmorafluorescentmicroscopewithCy5filter.
SpectrumAdvancedSpectrumViewer



Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.Preparecells:

1.1   Foradherentcells:Platecellsovernightingrowthmediumat10,000to40,000cells/well/90µLfora96-wellplateor2,500to10,000cells/well/20µLfora384-wellplate.

 

1.2   Fornon-adherentcells:CentrifugethecellsfromtheculturemediumandsUSPendthecellpelletsinculturemediumat50,000-100,000cells/well/90µLfora96-wellpoly-Dlysineplateor10,000-25,000cells/well/20µLfora384-wellpoly-Dlysineplate.Centrifugetheplateat800rpmfor2minuteswithbrakeoffpriortoyourexperiment.

Note:Eachcelllineshouldbeevaluatedonanindividualbasistodeterminetheoptimalcelldensity.

2.PrepareROSBrite™670AssaySolution:

2.1   PrepareROSBrite™670stocksolution(500X):Add40µLofDMSO(ComponentC)intothevialofROSBrite™670(ComponentA),andmixthemwell.

Note:20µLofreconstitutedROSBrite™670stocksolutionisenoughfor1plate.Unusedportioncanbealiquotedandstoredat<-20°Cformorethanonemonthifthetubesaresealedtightlyandkeptfromlight.Avoidrepeatedfreeze-thawcycles.

 

 

2.2  PrepareROSBrite™670assaysolution:Add20µLof500XDMSOreconstitutedROSBrite™670stocksolution(fromStep2.1)into10mLofAssayBuffer(ComponentB),andmixthemwell.Thisassaysolutionisstableforatleast2hoursatroomtemperature.

3.RunROSAssay:

3.1   Treatcellswith10μLof10Xtestcompounds(96-wellplate)or5μLof5Xtestcompounds(384-wellplate)inyourdesiredbuffer(suchasPBSorHHBS).Forcontrolwells(untreatedcells),addthecorrespondingamountofcompoundbuffer.

3.2   ToinduceROS,incubatethecellplateatroomtemperatureorina5%CO2,37°Cincubatorforadesiredperiodoftime(forexample:30minutestreatmentforHelacellswith100µMtert-butylhydroperoxide(TBHP)).

3.3   Add100µL/well(96-wellplate)or25µL/well(384-wellplate)ofROSBrite™670workingsolution(fromStep2.2)intothecellplate.

3.4   Incubatethecellsina5%CO2,37°Cincubatorfor30mintoonehour,andmonitorthefluorescenceincreaseatEx/Em=650/675nm(cutoff=665m)withbottomreadmode,ortakeimageswithTRITCfilterset.

References&Citations
CitationExplorer

Anti-proliferationeffectofbluelight-emittingdiodesagainstantibiotic-resistantHelicobacterpylori
Authors:JianweiMa,TakahiroHiratsuka,TsuyoshiEtoh,JunkoAkada,HajimeFujishima,NorioShiraishi,YoshioYamaoka,MasafumiInomata
Journal:JournalofGastroenterologyandHepatology(2017)

NotoginsenosideR1attenuateshighglucose-inducedendothelialdamageinratretinalcapillaryendothelialcellsbymodulatingtheintracellularredoxstate
Authors:ChunlanFan,YuanQiao,MinkeTang
Journal:DrugDesign,DevelopmentandTherapy(2017):3343

Goodhydrationandcell-biologicalperformancesofsuperparamagneticcalciumphosphatecementwithconcentration-dependentosteogenesisandangiogenesisinducedbyferriciron
Authors:JZhang,HSShi,JQLiu,TYu,ZHShen,JDYe
Journal:JournalofMaterialsChemistryB(2015):8782--8795

TopiramateProtectsPericytesfromGlucotoxicity:RoleforMitochondrialCAVAinCerebromicrovascularDiseaseinDiabetes
Authors:PingPatrick,TulinOPrice,AnaLDiogo,NaderSheibani,WilliamABanks,GulNShah
Journal:Journalofendocrinologyanddiabetes(2015)

Down-regulatedperoxisomeproliferator-activatedreceptorγ(PPARγ)inlungepithelialcellspromotesaPPARγagoNIST-reversIBLeproinflammatoryphenotypeinchronicobstructivepulmonarydisease(COPD)
Authors:SowmyaPLakshmi,AravindTReddy,YingzeZhang,FrankCSciurba,RamaKMallampalli,StevenRDuncan,RajuCReddy
Journal:JournalofBiologicalChemistry(2014):6383--6393

Superoxidedismutaseasatargetofclioquinol-inducedneurotoxicity
Authors:KazuyukiKawamura,YukikoKuroda,MasakoSogo,MikiFujimoto,ToshioInui,TakaoMitsui
Journal:Biochemicalandbiophysicalresearchcommunications(2014):181--185

Xanthineoxidaseinhibitionbyfebuxostatattenuatesexperimentalatherosclerosisinmice
Authors:JohjiNomura,NathalieBusso,AnnetteIves,ChiekoMatsui,SyunsukeTsujimoto,TakashiShirakura,MizuhoTamura,TsunefumiKobayashi,AlexanderSo,YoshihiroYamanaka
Journal:Scientificreports(2014):4554

Highglucose-inducedmitochondrialrespirationandreactiveoxygenspeciesinmousecerebralpericytesisreversedbypharmacologicalinhibitionofmitochondrialcarbonicanhydrases:implicationsforcerebralmicrovasculardiseaseindiabetes
Authors:GulNShah,YoichiMorofuji,WilliamABanks,TulinOPrice
Journal:Biochemicalandbiophysicalresearchcommunications(2013):354--358


品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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