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当前位置: 首页 > 产品中心 > Other_biological_dyes > AAT Bioquest/MyCLight™绿色JJ99/24001/100 ul
商品详细AAT Bioquest/MyCLight™绿色JJ99/24001/100 ul
AAT Bioquest/MyCLight™绿色JJ99/24001/100 ul
AAT Bioquest/MyCLight™绿色JJ99/24001/100 ul
商品编号: 24001
品牌: aatbio
市场价: ¥56560.00
美元价: 33936.00
产地: 美国(厂家直采)
公司:
产品分类: 其他生物染料
公司分类: Other_biological_dyes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Overview
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Ex/Em(nm)484/504
MWN/A
CAS#N/A
SolventDMSO
StorageF/D/L
CategoryMicroBIOLOGy
FlowCytometry
RelatedFluorescenceImaging
BiochemicalAssays
MycoLight™GreenJJ99stainisanexcellentgreen-fluorescentnuclearandchromosomecounterstainthatispermeanttobothprokaryoticandeukaryoticcellmembranes.MycoLight™GreenJJ99stainhasahighaffinityforDNAandexhibitsenhancedfluorescenceuponbindingwithanexcitationmaximumclosetothe488nmargonlaserlineandfluorescenceemissionmaximumat~500nm.MycoLight™GreenJJ99stainisparticularlyusefulasanuclearcounterstainforbacterialassayssinceitstainsbothliveanddeadGram-positiveandGram-negativebacteria.ItisanexcellentreplacementforSTYO®9(STYO®isthetrademarkofInvitrogen).
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
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Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

Note1:Thefollowingprotocolcanbeadaptedformostcelltypes.Theseconditionsrequireadjustmentforeachcelltypeandexperimentalsystem.Growthmedium,celldensity,thepresenceofothercelltypesandfactorsmayinfluencestaining.Residualdetergentonglasswaremayalsoaffectstainingofmanyorganisms,andcausebrightlystainedmaterialtoappearinsolutionswithorwithoutcellspresent.

Note2:UseplastictubeswhendilutingMycoLightTMGreenJJ98andJJ99,becausethedilutedstainadherestoglass.Ingeneral,thebestresultsareobtainedinbuffersthatdonotcontainphosphate.

 

1.Adherentcellsinculturemaybestainedinsituoncoverslips.PelletcellsinsUSPensionbycentrifugationand

   resuspendinbufferedsaltsolutionorwater.

2.DilutetheMycoLightTMGreenJJ98orJJ99withnon-phosphatebuffersuchasHepesbufferorbufferofyourchoice.Add MycoLightTMGreenJJ98.UsingtheconcentrationslistedbelowTable1asaguideline.Ininitialexperiments, itmaybebesttotryseveraldyeconcentrationsovertheentiresuggestedrangetodeterminetheconcentrationthat  yieldsoptimalstaining.

 

Table1.SuggestedconditionsforstainingcellswithMycoLightTMGreenJJ98

 

Application

Concentration

StainingConditions

Bacterialcells

50nM–20μM

Vortextomix,thenincubatefor1–30minutes.

Eukaryoticcells

10nM–5μM

Incubatefor10–120minutes.

Microarrays

50nMinTEbuffer

Incubatefor5minutes,rinseandthendry.

 

3.Stainedeukaryoticcellsgenerallyshowdiffusecytoplasmicstainingaswellasnuclearstaining.Particularly

   MycoLightTMGreenJJ98andJJ99intensestainingofintranuclearbodiesarefrequentlyobserved.

References&Citations
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1.   WattsMR,JamesG,SultanaY,GinnAN,OuthredAC,KongF,VerweijJJ,IredellJR,ChenSC,LeeR.(2014)Aloop-mediatedisothermalamplification(LAMP)assayforStrongyloidesstercoralisinstoolthatusesavisualdetectionmethodwithSYTO-82fluorescentdye.AmJTropMedHyg,90,306.

2.   WeidnerJ,CassensU,GohdeW,WullenweberJ,GreveB.(2013)AnewtriplexrealtimePCRwhichdistinguishesbetweenMRSA,MSSA,andmecAcoagulasenegativestrainsbymeansofmeltingpointanalysisusingSYTO9.ClinLab,59,795.

3.   HubbardKS,GutIM,ScheelerSM,LymanME,McNuttPM.(2012)CompatibilityofSYTO13andHoechst33342forlongitudinalimagingofneuronviABIlityandcelldeath.BMCResNotes,5,437.

4.   LarrosaM,TruchadoP,EspinJC,Tomas-BarberanFA,AllendeA,Garcia-ConesaMT.(2012)EvaluationofPseudomonasaeruginosa(PAO1)adhesiontohumanalveolarepithelialcellsA549usingSYTO9dye.MolCellProbes,26,121.

5.   EischeidAC.(2011)SYTOdyesandEvaGreenoutperformSYBRGreeninreal-timePCR.BMCResNotes,4,263.

6.   WlodkowicD,SkommerJ,DarzynkiewiczZ.(2011)RapidquantificationofcellviabilityandapoptosisinB-celllymphomaculturesusingcyanineSYTOprobes.MethodsMolBiol,740,81.

7.   SeputieneV,VilkoicaiteA,ArmalyteJ,PavilonisA,SuziedelieneE.(2010)Detectionofmethicillin-resistantStaphylococcusaureususingdoubleduplexreal-timePCRanddyeSyto9.FoliaMicrobiol(Praha),55,502.

8.   UllalAJ,PisetskyDS,ReichCF,3rd.(2010)UseofSYTO13,afluorescentdyebindingnucleicacids,forthedetectionofmicroparticlesininvitrosystems.CytometryA,77,294.

9.   UdovichJA,BesselsenDG,GmitroAF.(2009)AssessmentofacridineorangeandSYTO16forinvivoimagingoftheperitonealtissuesinmice.JMicrosc,234,124.

10.   WlodkowicD,SkommerJ,FaleyS,DarzynkiewiczZ,CooperJM.(2009)DynamicanalysisofapoptosisusingcyanineSYTOprobes:fromclassicaltomicrofluidiccytometry.ExpCellRes,315,1706.


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品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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