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当前位置: 首页 > 产品中心 > Other_biological_dyes > AAT Bioquest/CF-MUP,钠盐*优于MUP*/11628/10 mg
商品详细AAT Bioquest/CF-MUP,钠盐*优于MUP*/11628/10 mg
AAT Bioquest/CF-MUP,钠盐*优于MUP*/11628/10 mg
AAT Bioquest/CF-MUP,钠盐*优于MUP*/11628/10 mg
商品编号: 11628
品牌: aatbio
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 其他生物染料
公司分类: Other_biological_dyes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Overview
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Ex/Em(nm)360/450
MW352.55
CAS#N/A
SolventWater
StorageF/D/L
CategoryEnzymeDetection
Phosphatases
RelatedProteinPhosphatase
AlthoughMUPiswidelyusedfordetectingphosphatasesinsolutionitisnotwellsuitedforlivingcellorcontinuousassayssinceMU(4-methylumbelliferone),theenzymaticproduct,whichonlydevelopsmaximumfluorescenceatpHvalueof>10.ThusitisalsodifficulttouseMUPforthedetectionofphosphatasesthathaveacidicoptimalpHrangesuchasacidphosphatases.AATBioquestispleasedtoofferCF-MUPPlusthatisdevelopedtoaddressthispHlimitationassociatedwithMUPsubstrates.CF-MUexhibitsmaximumfluorescenceabovepH5.0,thusCF-MUPsubstratecanbewellusedforcontinuousphosphataseassays.ItcanalsobeusedfortheassaysthatrequireacidicpHsuchasacidphosphatasesandsomeproteinphosphatases.
SpectrumAdvancedSpectrumViewer

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Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.Prepareworkingsolution:

 

1.1   Preparea2to10mMstocksolutioninanappreciatesolvent(seeTable).Thestocksolutionshouldbeusedpromptly.Anyunusedsolutionneedtobealiquotedandfrozenat<-20oC.

Note1:DonotuseDMSO,ETOHorMETHtomakestocksolutionofSunRed™Phosphate(cat#11629)sinceitsignificantlyincreasesassaybackground.

Note2:Avoidrepeatedfreeze-thawcycles,andprotectfromlight.

1.2   Preparea2XPhosphateworkingsolution: Onthedayoftheexperiment,eitherdissolvethesubstrateinanappreciatesolventorthawanaliquotofthestocksolution(fromStep1.1)atroomtemperature.Preparea2Xworkingsolutionof10to50µMin100mMTrisbufferorbufferofyourchoice,pH8to9(notphosphatebuffer).

 

 

2.Runphosphataseassayinsupernatants:

 

2.1   Add50µLof2XPhosphateworkingsolution(fromStep1.2)intoeachwellofthephosphatasestandard,blankcontrol,andtestsamplestomakethetotalphosphataseassayvolumeof100µL/well.

Note:Fora384-wellplate,add25µLofsampleand25µLof2XPhosphateworkingsolutionintoeachwell.

 

2.2   Incubatethereactionfor30to120minutesatthedesiredtemperature,protectedfromlight.

 

2.3   Monitorthefluorescenceincreaseatanappropriatefiltersets(Seetable)withafluorescenceplatereader.

 

2.4   Thefluorescenceinblankwells(withtheassaybufferonly)isusedasacontrol,andissubtractedfromthevaluesforthosewellswiththephosphatasereactions.

References&Citations
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1.   BednarskaK,KlinkM,SulowskaZ.(2006)Applicationofintracellularalkalinephosphataseactivitymeasurementindetectionofneutrophiladherenceinvitro.MediatorsInflamm,2006,19307.

2.   WohlsenT,BaylissJ,GrayB,BatesJ,KatouliM.(2006)EvaluationofanalternativemethodfortheenumerationandconfirmationofClostridiumperfringensfromtreatedanduntreatedsewages.LettApplMicrobiol,42,438.

3.   AndersonBH,MagdoffFR.(2005)Relativemovementandsoilfixationofsolubleorganicandinorganicphosphorus.JEnvironQual,34,2228.

4.   RinconV,CorredorA,Martinez-GutierrezM,CastellanosJE.(2005)Fluorometriccell-ELISAforquantifyingrABIesinfectionandheparininhibition.JVirolMethods,127,33.

5.   HoehamerCF,MazurCS,WolfeNL.(2005)PurificationandpartialcharacterizationofanacidphosphatasefromSpirodelaoligorrhizaanditsaffinityforselectedorganophosphatepesticides.JAgricFoodChem,53,90.

6.   ShroyerML,BhuniaAK.(2003)Developmentofarapid1-hfluorescence-basedcytotoxicityassayforListeriaspecies.JMicrobiolMethods,55,35.

7.   ChruszczM,LaidlerP,MonkiewiczM,OrtlundE,LebiodaL,LewinskiK.(2003)CrystalstructureofacovalentintermediateofendogenoushumanarylsulfataseA.JInorgBiochem,96,386.

8.   BautersTG,PelemanR,DhontM,VanhaesebrouckP,NelisHJ.(2003)EnzymaticdifferentiationofCandidaparapsilosisfromotherCandidaspp.inamembranefiltrationtest.JMicrobiolMethods,53,11.

9.   BouaichaN,MaatoukI,VincentG,LeviY.(2002)Acolorimetricandfluorometricmicroplateassayforthedetectionofmicrocystin-LRindrinkingwaterwithoutpreconcentration.FoodChemToxicol,40,1677.

10.   BarkhudaryanN,GambarovS,GyulbayazyanT,NahapetyanK.(2002)LVV-hemorphin-4modulatesCa2+/calmodulin-dependentpathwaysintheimmunesystembythesamemechanismasinthebrain.JMolNeurosci,18,203.


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品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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