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当前位置: 首页 > 产品中心 > Other_biological_dyes > AAT Bioquest/Suc-LLVY-Aminoluciferin/13452/1 mg
商品详细AAT Bioquest/Suc-LLVY-Aminoluciferin/13452/1 mg
AAT Bioquest/Suc-LLVY-Aminoluciferin/13452/1 mg
AAT Bioquest/Suc-LLVY-Aminoluciferin/13452/1 mg
商品编号: 13452
品牌: aatbio
市场价: ¥56560.00
美元价: 33936.00
产地: 美国(厂家直采)
公司:
产品分类: 其他生物染料
公司分类: Other_biological_dyes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
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Ex/Em(nm)None/560
MW868.03
CAS#N/A
SolventDMSO
StorageF/D/L
CategoryEnzymeDetection
PeptidasesandProteases
RelatedBiochemicalAssays
Suc-LLVY-D-Aminolucifinisasentiveluminogenicsubstratefor20Sproteasome,calpainsandotherchymotrypsin-likeproteases.Upontheprotease-inducedhydrolysisthenon-luminescentsubstrategeneratestheluminescentD-aminoluciferinthatcanbeeasilydetectedwithluciferase-inducedluminescence.Thisaminoluciferinsubstrateismuchmoresensitivethanthechromogenicandfluorogenicsubstrates.Themostcommonformoftheproteasomeisknownasthe26Sproteasomethatcontainsone20Scoreparticlestructureandtwo19Sregulatorycaps.All20Sparticlesconsistoffourstackedheptamericringstructuresthatarethemselvescomposedoftwodifferenttypesofsubunits;alphasubunitsarestructuralinnature,whereasbetasubunitsarepredominantlycatalytic.Theoutertworingsinthestackconsistofsevenalphasubunitseach,whichserveasdockingdomainsfortheregulatoryparticlesandthealphasubunitsN-terminiformagatethatblocksunregulatedaccessofsubstratestotheinteriorcavity.Theinnertworingseachconsistofsevenbetasubunitsandcontaintheproteaseactivesitesthatperformtheproteolysisreactions.
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
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Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.      Preparea5to10mMstocksolutioninDMSO.

2.      Preparea2Xproteasomesubstrate(20to50µM)assaysolutionasthefollowing:

 

25to50µLsubstratestocksolution(10mM)

100µLDTT(1M)

400µLEDTA(100mM)

10mLHepesBuffer(25mM),pH=7.4

 

3.      Mixequalvolumeoftheprotesomestandardsorsampleswith2Xfluorescentproteasomesubstrateassaysolution(fromStep1),andincubatethesolutionsatroomtemperatureforatleast1hour.

 

4.      Monitorthefluorescenceusefluorescentmicroplatereaders.

References&Citations
CitationExplorer

Diabetogenicagentalloxanisaproteasomeinhibitor
Authors:WenjuanZhou,LinglingWei,TingXiao,ChunyouLai,MinPeng,LingliXu,XiangweiLuo,ShaopingDeng,FengxueZhang
Journal:BiochemicalandBiophysicalResearchCommunications(2017):400--406

DelineationofmolecularpathwaysinvolvedincardiomyopathiescausedbytroponinTmutations
Authors:JenniferEGilda,XianyinLai,FrankAWitzmann,AldrinVGomes
Journal:Molecular&CellularProteomics(2016):1962--1981

Diclofenacinducesproteasomeandmitochondrialdysfunctioninmurinecardiomyocytesandhearts
Authors:RajeshwaryGhosh,SumantaKGoswami,LuisFelipeBBFeitoza,BruceHammock,AldrinVGomes
Journal:InternationalJournalofCardiology(2016):923--935

Advanced-glycation-end-product-inducedformationofimmunoproteasomes:involvementofRAGEandJak2/STAT1
Authors:StefanieGrimm,ChristianeOtt,MelanieHörlacher,DanielaWeber,AnnikaHöhn,TilmanGrune
Journal:BiochemicalJournal(2012):127--139


品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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