| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | None/None |
| MW | 366.44 |
| CAS# | N/A |
| Solvent | DMSO |
| Storage | F/D |
| Category | BiotinandItsDerivatives Thiol-ReactiveBiotins |
| Related | Thiol-ReactiveProbes BiochemicalAssays |
SampleProtocolforLabelingProteinswithBiotinC2Maleimide:
1) Dissolveyourthiol-containingproteinatconcentrationof1-10mg/mL(3-10mgistheoptimallabelingconcentration)usingPBSbuffer(20mM,pH7.2).
2) DissolvetheBiotinC2MaleimideinDMSOatconcentrationof5-10mg/mL.
3) MixtheBiotinC2Maleimide(fromStep2)andproteinsolution(fromStep1)at2:1molarratioofbiotin/protein,andshakethereactionmixtureatroomtemperaturefor2-4hours.
4) Filterthereactionmixturethroughaproteinspincolumnfor100µgto1mgproteinlabelingreaction;orpurifytheconjugateusinggelfiltrationonaproperlysizedSephadexG-25columnifthereactionscaleislargerthan1mg.
5) Collectthedesiredfractionsforyourimmediateuseorfreezedrythemforyourfutureuse.
SampleProtocolforLabelingSmallMoleculeswithBiotinC2Maleimide:
1) DissolveBiotinC2Maleimide(10-15mg/mL)andyourthiol-containingmoleculeinDMSOat1:1.2molarratioofbiotin/thiol-containmolecule.
2) Stirthereactionmixtureatroomtemperaturefor2-4hours.
3) PurifytheconjugateusingHPLC(ammoniumacetate/waterandacetonitrile,pH7.0).
4) Collectandpoolthedesiredfractions.
5) Combineandfreeze-drythepooledfractions.
| References&Citations |  CitationExplorer |
Alow-cost,high-performancesystemforfluorescencelateralflowassays
Authors:LindaGLee,EricSNordman,MartinDJohnson,MarkFOldham
Journal:Biosensors(2013):360--373
ImplementationofP22viralcapsidsasnanoplatforms
Authors:SebyungKang,MasakiUchida,AlisonO’Neil,RuiLi,PeterEPrevelige,TrevorDouglas
Journal:Biomacromolecules(2010):2804--2809
Calcium-dependentactivationoftransglutaminase2bynanosecondpulsedelectricfields
Authors:KeikoMorotomi-Yano,Ken-ichiYano
Journal:FEBSOpenBio
