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AAT Bioquest/ReadiUse™ Preactivated APC-iFluor™ 750 Tandem/2571/1 mg
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 651/779 |
| MW | N/A |
| CAS# | N/A |
| Solvent | Water |
| Storage | R/D/L |
| Category | Protein-BasedTags Phycoproteins |
| Related | ProteinDyes |
| Spectrum | AdvancedSpectrumViewer |
- Pre-activateantibodywithBuccutite™MTA
- ReconstituteBuccutite™MTAinDMSOat~10mg/mL.
Note:PleasestoreunusedBuccutite™MTAat-20°Candcouldbeuseduptotwofreezeandthawcycles - Preparetargetantibody(Ab)inpH=8.5~9.0bufferatconcentrationabove1mg/ml.
- AddBuccutite™MTAtoAbsolutionattheratioof8~10µgBuccutite™MTA/100µgAb.
- MixwellandreactatRTfor60minutes,rotatingduringthereaction.
- PurifythereactionmixturewithdesaltingcolumntoremoveunreactedBuccutite™MTAandexchangebuffertoPBSorbufferofyourchoice.
- CollecttheBuccutite™MTA-activatedAb,andestimatetheconcentrationby70%yieldoftheoriginalstartingamount.
- ReconstituteBuccutite™MTAinDMSOat~10mg/mL.
- Conjugatewithpre-activatedAPC,APC-ifluors,orAPC-ifluortandems
- Reconstitutepre-activatedAPC,APC,APC-ifluors,orAPC-ifluortandemsin100µLddH2Oto10mg/mL.
Note:Reconstitutedpre-activatedAPC,APC-ifluors,orAPC-ifluortandemsarenotstableandcouldbestoredat4°Cforonemonth,keptfromlight. - AddAPC,APC-ifluors,orAPC-ifluortandemsdirectlytoMTA-activatedtargetAbsolution(from1.5)attheratioof130µgAPC,APC-ifluors,orAPC-ifluortandems/100µgMTA-activatedAb.
- Rotatethemixturefor1houratroomtemperature
- TheAb/APC,APC-ifluors,orAPC-ifluortandemsconjugatesarenowreadytouse.
Note1(Optional):Ab/APC,APC-ifluors,orAPC-ifluortandemsconjugatescouldbefurtherpurifiedthroughsizeexclusionchromatographytogetbestperformance.
Note2:Theantibodyconjugateshouldbestoredat>0.5mg/mLinthepresenceofacarrierprotein(e.g.,0.1%bovineserumalbumin)and0.02-0.05%sodiumazide.
Note3:TheAb/APC,APC-ifluors,orAPC-ifluortandemsconjugatessolutioncouldbestoredat4°Cforuptotwomonths,andkeptfromlight.
- Reconstitutepre-activatedAPC,APC,APC-ifluors,orAPC-ifluortandemsin100µLddH2Oto10mg/mL.
| References&Citations |  PrinterFriendlyVersion |
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