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主营:主营:研究并生产荧光和发光探针,信号转导研究的试剂
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当前位置: 首页 > 产品中心 > Other_biological_dyes > AAT Bioquest/ROS Brite™ 570 *Optimized for Detecting Reactive Oxygen Species (ROS)*/16000/1 mg
商品详细AAT Bioquest/ROS Brite™ 570 *Optimized for Detecting Reactive Oxygen Species (ROS)*/16000/1 mg
AAT Bioquest/ROS Brite™ 570 *Optimized for Detecting Reactive Oxygen Species (ROS)*/16000/1 mg
AAT Bioquest/ROS Brite™ 570 *Optimized for Detecting Reactive Oxygen Species (ROS)*/16000/1 mg
商品编号: 16000
品牌: aatbio
市场价: ¥56560.00
美元价: 33936.00
产地: 美国(厂家直采)
公司:
产品分类: 其他生物染料
公司分类: Other_biological_dyes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
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Ex/Em(nm)556/566
MW732.81
CAS#N/A
SolventDMSO
StorageF/D/L
CategoryNeuroBIOLOGy
ReactiveOxygenSpecies
RelatedCellSignaling
BiochemicalAssays
Reactiveoxygenspecies(ROS)arechemicallyreactivemoleculescontainingoxygen.Examplesincludesuperoxide,hydroxylrADIcal,singletoxygenandperoxides.ROSishighlyreactiveduetothepresenceofunpairedvalenceshellelectrons.ROSformsasanaturalbyproductofthenormalmetabolismofoxygenandhaveimportantrolesincellsignalingandhomeostasis.However,duringtimesofenvironmentalstress(e.g.,UVorheatexposure),ROSlevelscanincreasedramatically.Thismayresultinsignificantdamagetocellstructures.Cumulatively,thisisknownasoxidativestress.ROSarealsogeneratedbyexogenoussourcessuchasionizingradiation.Underconditionsofoxidativestress,ROSproductionisdramaticallyincreased,resultinginsubsequentalterationofmembranelipids,proteins,andnucleicacids.Oxidativedamageofthesebiomoleculesisassociatedwithagingaswellaswithavarietyofpathologicalevents,includingatherosclerosis,carcinogenesis,ischemicreperfusioninjury,andneurodegenerativedisorders.ROSBrite™570reagentisanewfluorogenicprobetomeasureoxidativestressincellsusingconventionalfluorescencemicroscopy,high-contentimaging,microplatefluorometry,orflowcytometry.Thecell-permeantROSBrite™570reagentisnonfluorescentandproducesbrightorangefluorescenceuponROSoxidation.Theresultingfluorescencecanbemeasuredusingfluorescenceimaging,high-contentimaging,microplatefluorometry,orflowcytometry.
SpectrumAdvancedSpectrumViewer



Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1)      Preparea10to20mMROSBrite™stocksolutioninDMSO.Make5to10µMworkingsolutionbydilutingtheDMSOstocksolutionintoHankssolutionwith20mMHepesbuffer(HHBS).

2)      Treatcellsasdesired(e.g.,RASMcellsaretreatedwith50-100nMangiotensinIIfor3-5hours).

3)      IncubatethecellswithROSBrite™(5-10µM,fromStep#1)for15-30minutesat37ºC.

Note:TheconcentrationofROSBrite™usedvarieswithdifferentcelllines,onewillneedtestwithdifferentconcentrationstogettheoptimaldose.Forcat#16053,onemightuselessconcentrationsuchas0.5-5uM.

4)      Replacethedye-loadingsolutionwithHHBSbuffer.

5)      Analyzethecellswithaproperfluorescenceinstrument(e.g.,afluorescencemicroscope,flowcytometeroraninvivoimaginginstrument).

References&Citations
CitationExplorer

Thiol-MediatedSynthesisofHyaluronicAcid-Epigallocatechin-3-O-GallateConjugatesfortheFormationofInjectableHydrogelswithFreeRadicalScavengingPropertyandDegradationResistance
Authors:ChixuanLiu,KiHyunBae,AtsushiYamashita,JooEunChung,MotoichiKurisawa
Journal:Biomacromolecules(2017)

Transientreceptorpotentialmelastatin8ionchannelinmacrophagesmodulatescolitisthroughabalance-shiftinTNF-alphaandinterleukin-10production
Authors:MKhalil,ABabes,RLakra,SFörsch,PWReeh,SWirtz,CBecker,MFNeurath,MAEngel
Journal:Mucosalimmunology(2016)

VEGFR2signalingpreventscolorectalcancercellsenescencetopromotetumOrigenesisinmicewithcolitis
Authors:SebastianFoersch,TobiasSperka,ChristinaLindner,AstridTaut,KarlLRudolph,GeorgBreier,FrankBoxberger,TilmanTRau,ArndtHartmann,MichaelStürzl
Journal:Gastroenterology(2015):177--189


品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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