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当前位置: 首页 > 产品中心 > Other_biological_dyes > AAT Bioquest/FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK/13417/100 ug
商品详细AAT Bioquest/FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK/13417/100 ug
AAT Bioquest/FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK/13417/100 ug
AAT Bioquest/FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK/13417/100 ug
商品编号: 13417
品牌: aatbio
市场价: ¥56560.00
美元价: 33936.00
产地: 美国(厂家直采)
公司:
产品分类: 其他生物染料
公司分类: Other_biological_dyes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Overview
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Ex/Em(nm)492/516
MW1037.07
CAS#N/A
SolventDMSO
StorageF/D/L
CategoryEnzymeDetection
PeptidasesandProteases
RelatedCellApoptosis
ApoptosisandCytotoxicity
BiochemicalAssays
Activationofcaspasesplaysacentralroleinapoptosis.FITC-C6-D(OMe)E(OMe)VD(OMe)-FMKprovidesaconvenientmeansforsensitivedetectionofactivatedcaspase-3inlivingcells.FITC-C6-D(OMe)E(OMe)VD(OMe)-FMKiscellpermeable,nontoxic,andirreversIBLybindstoactivatedcaspase-3inapoptoticcells.Basedonourin-housestudiesFITC-C6-D(OMe)E(OMe)VD(OMe)-FMKgavethehighestsignal/backgroundratiocomparedtootherDEVD-FMKprobeswetested.TheFITClabelallowsfordirectdetectionofactivatedcaspasesinapoptoticcellsbyfluorescencemicroscopy,flowcytometry,orfluorescenceplatereader.
SpectrumAdvancedSpectrumViewer

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Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.      GeneralSolutionCaspaseAssaysUsingAMC,AFC,pNA,R110andProRedSubstrates

1.1.    Preparea10mMstocksolutioninDMSO.

1.2.    Preparea2Xcaspase substrate(50µM)assaysolutionasthefollowing:

50µLsubstratestocksolution

100 µLDTT(1M)

400 µLEDTA(100mM)

10mLTrisBuffer(20mM),pH=7.4

1.3.    Mixequalvolumeofthecaspasestandardsorsampleswith2Xcaspasesubstrateassaysolution(fromStep1.2),andincubatethesolutionsatroomtemperatureforatleast1hour.

1.4.    Monitorthefluorescenceusingafluorescencemicroplatereader,orabsorbanceusinganabsorbancemicroplatereader.

2.      CellCaspaseAssaysUsingCell-PermeableFMKCaspaseProbes

2.1.    Preparea2-5mMstocksolutioninDMSO.

2.2.    Treatcellsasdesired.

2.3.    Preparea2Xpermeablecaspasesubstrate(20µM)assaysolutionbydilutingtheDMSOstocksolution(fromStep2.1)inHankswith20mMHepesbuffer(HHBS).

2.4.    Mixequalvolumeofthetreatedcellswith2Xcaspasesubstrateassaysolution(fromStep2.3),andincubatethecellsina37°C,5%CO2incubatorforatleast1hour.

2.5.    WashthecellswithHHBSforatleastonce.

2.6.    Monitorthefluorescenceintensitybyaflowcytometer,afluorescencemicroscopeorafluorescencemicroplatereader.

3.      CellCaspaseAssaysUsingCell-PermeableFMKCaspaseProbes(For#13470-13476only)

3.1.    Preparea250Xstocksolutionbyadding50µLDMSOintothevial.

3.2.    Treatcellsasdesired.

3.3.    Add250XDMSOstocksolution(fromStep3.1)intothecellsolutionata1:250ratio(suchas2uLto500uLcells),andincubatethecellsina37°C,5%CO2incubatorfor1hour.

3.4.    WashthecellswithHHBSforatleastonce.

3.5.    Monitorthefluorescenceintensitybyflowcytometer,fluorescencemicroscopyorfluorescentmicroplatereader.

References&Citations
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1.   LiJN,SongDQ,JiangJD.(2004)[Antitumormechanismof3-bromopropionylaminobenzoylureaonleukemiaandlymphoma].YaoXueXueBao,39,491.

2.   ThraneC,KaufmannU,StummannBM,OlssonS.(2004)Activationofcaspase-likeactivityandpoly(ADP-ribose)polymerasedegradationduringsporulationinAspergillusnidulans.FungalGenetBiol,41,361.

3.   PandeyS,SmithB,WalkerPR,SikorskaM.(2000)Caspase-dependentandindependentcelldeathinrathepatoma5123tccells.Apoptosis,5,265.

4.   HashiramotoA,SanoH,MaekawaT,KawahitoY,KimuraS,KusakaY,WilderRL,KatoH,KondoM,NakajimaH.(1999)C-mycantisenseoligodeoxynucleotidescaninduceapoptosisanddown-regulateFasexpressioninrheumatoidsynoviocytes.ArthritisRheum,42,954.


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品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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