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AAT Bioquest/ROS Brite™ APF *Optimized for Detecting Reactive Oxygen Species (ROS)*/16050/1 mg
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 492/515 |
| MW | 423.42 |
| CAS# | N/A |
| Solvent | DMSO |
| Storage | F/D/L |
| Category | NeuroBIOLOGy ReactiveOxygenSpecies |
| Related | CellSignaling BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
1) Preparea10to20mMROSBrite™APF(orHPF)stocksolutioninDMSO.Make1to10µMworkingsolutionbydilutingtheDMSOstocksolutionintoHankssolutionwith20mMHepesbuffer(HHBS).
2) Treatcellsasdesired(e.g.,RASMcellsaretreatedwith50-100nMangiotensinIIfor3-5hours)
3) IncubatethecellswithROSBrite™APF(orHPF) (1-10µM,fromStep#1)for20-60minutesat37ºC.
4) Replacethedye-loadingsolutionwithHHBSbuffer.
5) AnalyzethecellswithaproperfluorescenceinstrumentatEx/Em=490/525mm(cutoff=515nM)withbottomreadmode(e.g.,FITCfiltersetforafluorescencemicroscope,FL1filterforaflowcytometer).
Note:BSAandphenolredcanaffectthefluorescenceandshouldbeusedwithcaution.BothAPFandHPFcanbeusedinsolutionassaysorforintracellularmeasurements.
| References&Citations |  PrinterFriendlyVersion |
1. DuM,ZhangD,YanC,ZhangX.(2012)Developmentaltoxicityevaluationofthreehexabromocyclododecanediastereoisomersonzebrafishembryos.AquatToxicol,112-113,1.
2. ParkWH.(2012)MAPKinhibitorsandsiRNAsdifferentiallyaffectcelldeathandROSlevelsinarsenictrioxide-treatedhumanpulmonaryfibroblastcells.OncolRep,27,1611.
3. ParkWH,KimSH.(2012)MG132,aproteasomeinhibitor,induceshumanpulmonaryfibroblastcelldeathviaincreasingROSlevelsandGSHdepletion.OncolRep,27,1284.
4. WuYT,LinCY,TsaiMY,ChenYH,LuYF,HuangCJ,ChengCM,HwangSP.(2011)beta-Lapachoneinducesheartmorphogeneticandfunctionaldefectsbypromotingthedeathoferythrocytesandtheendocardiuminzebrafishembryos.JBiomedSci,18,70.
5. YouBR,KimSZ,KimSH,ParkWH.(2011)Gallicacid-inducedlungcancercelldeathisaccompaniedbyROSincreaseandglutathionedepletion.MolCellBiochem,357,295.
6. ParkWH.(2011)MAPKinhibitorsdifferentiallyaffectgallicacid-inducedhumanpulmonaryfibroblastcellgrowthinhibition.MolMedReport,4,193.
7. ParkWH.(2011)Mitogen-activatedproteinkinaseinhibitorsdifferentlyaffectthegrowthinhibitionanddeathofaproteasomeinhibitor,MG132-treatedhumanpulmonaryfibroblastcells.HumExpToxicol,30,1945.
8. YouBR,ParkWH.(2011)ProteasomeinhibitionbyMG132inducesgrowthinhibitionanddeathofhumanpulmonaryfibroblastcellsinacaspase-independentmanner.OncolRep,25,1705.
9. TauraA,KikkawaYS,NakagawaT,ItoJ.(2010)Hydrogenprotectsvestibularhaircellsfromfreeradicals.ActaOtolaryngolSuppl,95.
10. YouBR,ParkWH.(2011)Gallicacid-inducedhumanpulmonaryfibroblastcelldeathisaccompaniedbyincreasesinROSlevelandGSHdepletion.DrugChemToxicol,34,38.
