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AAT Bioquest/MitoROS™ 580 *Optimized for Detecting Reactive Oxygen Species (ROS) in Mitochondria*/16052/500 Tests
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 510/580 |
| MW | N/A |
| CAS# | N/A |
| Solvent | DMSO |
| Storage | F/D/L |
| Category | NeuroBIOLOGy ReactiveOxygenSpecies |
| Related | CellSignaling BiochemicalAssays |
- Prepare1000XMitoROS™580DMSOstocksolutionbydissolvethecontentsofonevialofMitoROS™580mitochondrialsuperoxideindicatorin13μLofDMSO.
- Prepare2XMitoROS™580workingsolutionbydilutingtheDMSOstocksolutionintoHankssolutionwith20mMHepesbuffer(HHBS).
Note:The2XMitoROS™580workingsolutionisnotstable,useitpromptly - Treatcellsasdesired.
- Incubatethecells(suchas100µL/wellin96-wellplate)withequalvolumeof2XMitoROS™580workingsolutionfor10-30minutesat37°C,protectedfromlight.
Note1:ThefinalincellconcentrationoftheMitoROS™580shouldnotexceed1X.Concentrationsexceeding1Xcanproducecytotoxiceffects,includingalteredmitochondrialmorphologyandredistributionoffluorescencetonucleiandthecytosol.
Note:DifferentcellsreacttoMitoROS™580differently,adjusttheworkingconcentrationaccordingly. - WashcellsgentlythreetimesandreplaceitwithHHBSbuffer
- Analyzethecellswithaproperfluorescenceinstrument(e.g.,afluorescencemicroscope,flowcytometer)withEx/Em=510/580nm.
| References&Citations |  PrinterFriendlyVersion |
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5. BurtonLJ,BarnettP,SmithB,ArnoldRS,HudsonT,KunduK,MurthyN,Odero-MarahVA.(2014)Muscadinegrapeskinextractrevertssnail-mediatedepithelialmesenchymaltransitionviasuperoxidespeciesinhumanprostatecancercells.BMCComplementAlternMed,14,97.
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7. Chandra-KuntalK,LeeJ,SinghSV.(2013)Criticalroleforreactiveoxygenspeciesinapoptosisinductionandcellmigrationinhibitionbydiallyltrisulfide,acancerchemopreventivecomponentofgarlic.BreastCancerResTreat,138,69.
8. DamAD,MitchellAS,QuadrilateroJ.(2013)Inductionofmitochondrialbiogenesisprotectsagainstcaspase-dependentandcaspase-independentapoptosisinL6myoblasts.BiochimBiophysActa,1833,3426.
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