| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 560/574 |
| MW | 701.50 |
| CAS# | N/A |
| Solvent | DMSO |
| Storage | F/D/L |
| Category | NeuroBIOLOGy ReactiveOxygenSpecies |
| Related | CellSignaling BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
1) Preparea10to20mMROSBrite™stocksolutioninDMSO.Make5to10µMworkingsolutionbydilutingtheDMSOstocksolutionintoHankssolutionwith20mMHepesbuffer(HHBS).
2) Treatcellsasdesired(e.g.,RASMcellsaretreatedwith50-100nMangiotensinIIfor3-5hours).
3) IncubatethecellswithROSBrite™(5-10µM,fromStep#1)for15-30minutesat37ºC.
Note:TheconcentrationofROSBrite™usedvarieswithdifferentcelllines,onewillneedtestwithdifferentconcentrationstogettheoptimaldose.Forcat#16053,onemightuselessconcentrationsuchas0.5-5uM.
4) Replacethedye-loadingsolutionwithHHBSbuffer.
5) Analyzethecellswithaproperfluorescenceinstrument(e.g.,afluorescencemicroscope,flowcytometeroraninvivoimaginginstrument).
| References&Citations |  PrinterFriendlyVersion |
1. AitkenRJ,SmithTB,LordT,KuczeraL,KoppersAJ,NaumovskiN,ConnaughtonH,BakerMA,DeIuliisGN.(2013)Onmethodsforthedetectionofreactiveoxygenspeciesgenerationbyhumanspermatozoa:analysisofthecellularresponsestocatecholoestrogen,lipidaldehyde,menadioneandarachidonicacid.Andrology,1,192.
2. AmaralS,RedmannK,SanchezV,MallidisC,Ramalho-SantosJ,SchlattS.(2013)UVBirradiationasatooltoassessROS-induceddamageinhumanspermatozoa.Andrology,1,707.
3. BarbonettiA,VassalloMR,CinqueB,FilipponiS,MastromarinoP,CifoneMG,FrancavillaS,FrancavillaF.(2013)SolubleproductsofEscherichiacoliinducemitochondrialdysfunction-relatedspermmembranelipidperoxidationwhichispreventedbylactobacilli.PLoSOne,8,e83136.
4. BarbonettiA,VassalloMR,DiRosaA,LeombruniY,FelzaniG,GandiniL,LenziA,NecozioneS,FrancavillaS,FrancavillaF.(2013)Involvementofmitochondrialdysfunctionintheadverseeffectexertedbyseminalplasmafrommenwithspinalcordinjuryonspermmotility.Andrology,1,456.
5. BurtonLJ,BarnettP,SmithB,ArnoldRS,HudsonT,KunduK,MurthyN,Odero-MarahVA.(2014)Muscadinegrapeskinextractrevertssnail-mediatedepithelialmesenchymaltransitionviasuperoxidespeciesinhumanprostatecancercells.BMCComplementAlternMed,14,97.
6. CaleareAdeO,Lazarin-BidoiaD,CortezDA,Ueda-NakamuraT,DiasFilhoBP,SilvaSdeO,NakamuraCV.(2013)Trypanocidalactivityof1,3,7-trihydroxy-2-(3-methylbut-2-enyl)-xanthoneisolatedfromKielmeyeracoriacea.ParasitolInt,62,405.
7. Chandra-KuntalK,LeeJ,SinghSV.(2013)Criticalroleforreactiveoxygenspeciesinapoptosisinductionandcellmigrationinhibitionbydiallyltrisulfide,acancerchemopreventivecomponentofgarlic.BreastCancerResTreat,138,69.
8. DamAD,MitchellAS,QuadrilateroJ.(2013)Inductionofmitochondrialbiogenesisprotectsagainstcaspase-dependentandcaspase-independentapoptosisinL6myoblasts.BiochimBiophysActa,1833,3426.
9. DavisDA,AkopianG,WalshJP,SioutasC,MorganTE,FinchCE.(2013)UrbanairpollutantsreducesynapticfunctionofCA1neuronsviaanNMDA/NOpathwayinvitro.JNeuRochem,127,509.
10. HaraH,ArayaJ,ItoS,KobayashiK,TakasakaN,YoshiiY,WakuiH,KojimaJ,ShimizuK,NumataT,KawaishiM,KamiyaN,OdakaM,MorikawaT,KanekoY,NakayamaK,KuwanoK.(2013)Mitochondrialfragmentationincigarettesmoke-inducedbronchialepithelialcellsenescence.AmJPhysiolLungCellMolPhysiol,305,L737.
