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AAT Bioquest/7-AAD [7-Aminoactinomycin D] *CAS 7240-37-1*/17501/1 mg
![AAT Bioquest/7-AAD [7-Aminoactinomycin D] *CAS 7240-37-1*/17501/1 mg](https://www.ebiomall.cn/images/AAT201712/chemical-structure-for-7-aad-7-aminoactinomycin-d-cas-7240-37-1.png)
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 546/647 |
| MW | 1270.43 |
| CAS# | 7240-37-1 |
| Solvent | DMSO |
| Storage | F/D/L |
| Category | NucleicAcidDetection DNADetection |
| Related | LabelingCells FlowCytometry BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
7-AADstainingisnormallyperformedafterallotherstainings.Itstainsdeadcellsonly.Thefollowingprocedurecanbeadaptedformostcelltypes.Growthmedium,celldensity,thepresenceofothercelltypesandotherfactorsmayinfluencestaining.Residualdetergentonglasswaremayalsoaffectrealorapparentstainingofmanyorganisms,causingbrightlystainedmaterialtoappearinsolutionswithorwithoutcellspresent.
1).Make1-10mMDMSOstocksolution.TheDMSOstocksolutionisgoodfor6monthsifstoredat-20°C.
2).Usethefixationprotocolappropriateforyoursample.
3).PelletcellsbycentrifugationandresUSPendthecellsinbufferedsaltsolutionsormedia,withoptimaldyestainingatpH7.4.Adherentcellsinculturemaybestainedinsituoncoverslipsorinthecellculturewells.
4).Add7-AADstainusingtheconcentrationsfrom0.5to5µMandincubateitfor15to60minutesasaguide.
Note:Ininitialexperiments,itmaybebesttotryseveraldyeconcentrationsovertheentiresuggestedrangetodeterminetheconcentrationthatyieldsoptimalstaining.
5).MeasurethefluorescenceintensityatEx/Em=545/650nm(useFL3forflowcytometricanalysis)
CAUTION:7-AADisapotentialcarcinogen.Itisrecommendedthattheuserweargloves,protectiveclothing,andeye/faceprotectioninordertoavoidcontactwithskinandeyes.
| References&Citations |  CitationExplorer |
Resources-ApplicationNotesPhenotypicandEpigeneticMechanismofActionDeterminationsofHistoneMethylaseandDemethylaseInhibitorsusingDigitalWidefieldMicroscopy
Authors:BradLarson,PeterBanks
Journal:ExperimentalBIOLOGy(2017)
