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AAT Bioquest/DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*/17511/100 mg
![AAT Bioquest/DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*/17511/100 mg](https://www.ebiomall.cn/images/AAT201712/chemical-structure-for-dapi-4-6-diamidino-2-phenylindole-dihydrochloride-cas-28718-90-3.png)
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 358/461 |
| MW | 350.25 |
| CAS# | 28718-90-3 |
| Solvent | Water |
| Storage | F/D/L |
| Category | NucleicAcidDetection DNADetection |
| Related | LabelingCells FluorescenceImaging BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
Usethefixationprotocolappropriateforyoursample.DAPIstainingisnormallyperformedafterallotherstaining.
Thefollowingprocedurecanbeadaptedformostcelltypes.Growthmedium,celldensity,thepresenceofothercelltypesandotherfactorsmayinfluencestaining.Residualdetergentonglasswaremayalsoaffectrealorapparentstainingofmanyorganisms,causingbrightlystainedmaterialtoappearinsolutionswithorwithoutcellspresent.
PelletcellsbycentrifugationandresUSPendthecellsinbufferedsaltsolutionsormedia,withoptimaldyebindingatpH7.4.Adherentcellsinculturemaybestainedinsituoncoverslipsorinthecellculturewells.AddDAPIstainusingtheconcentrationsbetween0.5and5µMandincubateitfor15to60minutesasaguide.Ininitialexperiments,itmaybebesttotryseveraldyeconcentrationsovertheentiresuggestedrangetodeterminetheconcentrationthatyieldsoptimalstaining.
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