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主营:主营:研究并生产荧光和发光探针,信号转导研究的试剂
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当前位置: 首页 > 产品中心 > Other_biological_dyes > AAT Bioquest/DAPI Dilactate/17509/25 mg
商品详细AAT Bioquest/DAPI Dilactate/17509/25 mg
AAT Bioquest/DAPI Dilactate/17509/25 mg
AAT Bioquest/DAPI Dilactate/17509/25 mg
商品编号: 17509
品牌: aatbio
市场价: ¥27560.00
美元价: 16536.00
产地: 美国(厂家直采)
公司:
产品分类: 其他生物染料
公司分类: Other_biological_dyes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Overview
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Ex/Em(nm)358/461
MW457.48
CAS#N/A
SolventWater
StorageF/D/L
CategoryNucleicAcidDetection
DNADetection
RelatedLabelingCells
FluorescenceImaging
BiochemicalAssays
DAPIisafluorescentstainthatbindsstronglytoDNA.Itisusedextensivelyinfluorescencemicroscopy.SinceDAPIpassesthroughanintactcellmembrane,itcanbeusedtostainlivecellsbesidesfixedcells.Forfluorescencemicroscopy,DAPIisexcitedwithultravioletlight.Whenboundtodouble-strandedDNAitsabsorptionmaximumisat358nmanditsemissionmaximumisat461nm.OnedrawbackofDAPIisthatitsemissionisfairlybroad.DAPIalsobindstoRNAalthoughitisnotasstronglyfluorescentasitbindstoDNA.Itsemissionshiftstoaround500nmwhenboundtoRNA.DAPI"sblueemissionisconvenientformultiplexingassayssincethereisverylittlefluorescenceoverlapbetweenDAPIandgreen-fluorescentmoleculeslikefluoresceinandgreenfluorescentprotein(GFP),orred-fluorescentstainslikeTexasRed.Besideslabelingcellnuclei,DAPIisalsousedforthedetectionofmycoplasmaorvirusDNAincellcultures.DAPIdilactatehasimprovedwatersolubilitycomparedtoDAPIHClsalt.
SpectrumAdvancedSpectrumViewer

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References&Citations
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1.   TsuboiM,BenevidesJM,ThomasJrGJ.(2006)TheComplexofEthidiumBromidewithGenomicDNA:StructureAnalysisbyPolarizedRamanSpectroscopy.BiophysJ.

2.   SanchezM,BondanEF,LalloMA,SinhoriniIL,DagliML,MaiorkaPC,PereiraLA,GracaDL.(2006)[ImmunohistochemicalstainingofthemacrophagicandastrocyticresponseinthebrainstemofWistarratssubmittedtotheethidiumbromidegliotoxicmodelandtreatedwithcyclophosphamide].ArqNeuropsiquiatr,64,787.

3.   ByrneCD,deMelloAJ.(1998)Photophysicsofethidiumbromidecomplexedtoct-DNA:amaximumentropystudy.BiophysChem,70,173.

4.   HeinI,FleknaG,WagnerM,NockerA,CamperAK.(2006)PossibleerrorsintheinterpretationofethidiumbromideandPicoGreenDNAstainingresultsfromethidiummonoazide-treatedDNA.ApplEnvironMicrobiol,72,6860.

5.   BaskicD,PopovicS,RisticP,ArsenijevicNN.(2006)Analysisofcycloheximide-inducedapoptosisinhumanleukocytes:FluorescencemicroscopyusingannexinV/propidiumiodideversusacridinorange/ethidiumbromide.CellBiolInt,30,924.

6.   MazzantiCM,SpanevelloRM,PereiraLB,GoncalvesJF,KaizerR,CorreaM,AhmedM,MazzantiA,FestugattoR,GracaDL,MorschVM,SchetingerMR.(2006)Acetylcholinesteraseactivityinratsexperimentallydemyelinatedwithethidiumbromideandtreatedwithinterferonbeta.NeuRochemRes,31,1027.

7.   FachinAL,Ferreira-NozawaMS,MaccheroniW,Jr.,Martinez-RossiNM.(2006)RoleoftheABCtransporterTruMDR2interbinafine,4-nitroquinolineN-oxideandethidiumbromidesusceptibilityinTrichophytonrubrum.JMedMicrobiol,55,1093.

8.   AlonsoA,AlmendralMJ,CurtoY,CriadoJJ,RodriguezE,ManzanoJL.(2006)DeterminationoftheDNA-bindingcharacteristicsofethidiumbromide,proflavine,andcisplatinbyflowinjectionanalysis:usefulnessinstudiesonantitumordrugs.AnalBiochem,355,157.

9.   MazzantiCM,SpanevelloRM,ObregonA,PereiraLB,StreherCA,AhmedM,MazzantiA,GracaDL,MorschVM,SchetingerMR.(2006)Ethidiumbromideinhibitsratbrainacetylcholinesteraseactivityinvitro.ChemBiolInteract,162,121.

10.   TamVK,LiuQ,TorY.(2006)Extendedethidiumbromideanalogueasatriplehelixintercalator:synthesis,photophysicalpropertiesandnucleicacidsbinding.ChemCommun(Camb),2684.


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品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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