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AAT Bioquest/Propidium iodide *CAS 25535-16-4*/17515/25 mg
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 535/617 |
| MW | 668.39 |
| CAS# | 25535-16-4 |
| Solvent | DMSO |
| Storage | F/D/L |
| Category | NucleicAcidDetection DNADetection |
| Related | LabelingCells FluorescenceImaging BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
PIstainingisnormallyperformedafterallotherstainings.Itstainsdeadcellsonly.Thefollowingprocedurecanbeadaptedformostcelltypes.Growthmedium,celldensity,thepresenceofothercelltypesandotherfactorsmayinfluencestaining.Residualdetergentonglasswaremayalsoaffectrealorapparentstainingofmanyorganisms,causingbrightlystainedmaterialtoappearinsolutionswithorwithoutcellspresent.
1).Make1-10mMDMSOstocksolution.TheDMSOstocksolutionisgoodfor6monthsifstoredat-20°C.
2).Usethefixationprotocolappropriateforyoursample.
3).PelletcellsbycentrifugationandresUSPendthecellsinbufferedsaltsolutionsormedia,withoptimaldyestainingatpH7.4.Adherentcellsinculturemaybestainedinsituoncoverslipsorinthecellculturewells.
4).AddPIstainusingtheconcentrationsbetween0.5and5µMandincubateitfor15to60minutesasaguide.
Note:Ininitialexperiments,itmaybebesttotryseveraldyeconcentrationsovertheentiresuggestedrangetodeterminetheconcentrationthatyieldsoptimalstaining.
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