| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 352/461 |
| MW | 533.88 |
| CAS# | 23491-45-4 |
| Solvent | Water |
| Storage | F/L |
| Category | NucleicAcidDetection DNADetection |
| Related | LabelingCells FluorescenceImaging BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
Thefollowingprocedurecanbeadaptedformostcelltypes.Growthmedium,celldensity,thepresenceofothercelltypesandotherfactorsmayinfluencestaining.Residualdetergentonglasswaremayalsoaffectrealorapparentstainingofmanyorganisms,causingbrightlystainedmaterialtoappearinsolutionswithorwithoutcellspresent.
PelletcellsbycentrifugationandresUSPendthecellsinbufferedsaltsolutionsormedia,withoptimaldyebindingatpH7.4.Adherentcellsinculturemaybestainedinsituoncoverslipsorinthecellculturewells.AddHoechststainusingtheconcentrationsbetween0.5and5µMandincubateitfor15to60minutesasaguide.Ininitialexperiments,itmaybebesttotryseveraldyeconcentrationsovertheentiresuggestedrangetodeterminetheconcentrationthatyieldsoptimalstaining.
| References&Citations |  PrinterFriendlyVersion |
1. StreltsovSA,GromykoAV,OleinikovVA,ZhuzeAL.(2006)TheHoechst33258CovalentDimerCoversaTotalTurnoftheDouble-strandedDNA.JBiomolStructDyn,24,285.
2. WillisB,AryaDP.(2006)RecognitionofB-DNAbyneomycin--Hoechst33258conjugates.Biochemistry,45,10217.
3. CorreaBJ,CanzioD,KahaneAL,ReddyPM,BruiceTC.(2006)DNAsequencerecognitionbyHoechst33258conjugatesofhairpinpyrrole/imidazolepolyamides.BioorgMedChemLett,16,3745.
4. ChoiYS,ChoTS,KimJM,HanSW,KimSK.(2006)AmineterminatedG-6PAMAMdendrimeranditsinteractionwithDNAprobedbyHoechst33258.BiophysChem,121,142.
5. TanadaM,TsujitaS,SasakiS.(2006)DesignofnewbidentateligandsconstructedoftwoHoechst33258unitsfordiscriminationofthelengthoftwoA3T3bindingmotifs.JOrgChem,71,125.
6. KiserJR,MonkRW,SmallsRL,PettyJT.(2005)HydrationchangesintheassociationofHoechst33258withDNA.Biochemistry,44,16988.
7. ReddyPM,ToporowskiJW,KahaneAL,BruiceTC.(2005)Recognitionofa10basepairsequenceofDNAandstereochemicalcontrolofthebindingaffinityofchiralhairpinpolyamide-Hoechst33258conjugates.BioorgMedChemLett,15,5531.
8. GromykoAV,PopovKV,MozolevaAP,Strel"tsovSA,GrokhovskiiSL,OleinikovVA,ZhuzeAL.(2005)[LigandswithaffinitytospecificsequenceofDNAbasepairs.XII.ThesynthesisandcytologicalstudiesofdimericHoechst33258molecules].BioorgKhim,31,385.
9. HanF,TaulierN,ChalikianTV.(2005)AssociationoftheminorgroovebindingdrugHoechst33258withd(CGCGAATTCGCG)2:volumetric,calorimetric,andspectroscopiccharacterizations.Biochemistry,44,9785.
10. BielawskiK,BielawskaA,AnchimT,WolczynskiS.(2005)Synthesis,DNAbinding,topoisomeraseinhibitionandcytotoxicpropertiesof2-chloroethylnitrosoureaderivativesofhoechst33258.BiolPharmBull,28,1004.
