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当前位置: 首页 > 产品中心 > Other_biological_dyes > AAT生物探索/Hoechst 33342*CAS 23491-52-3*/17530/100毫克
商品详细AAT生物探索/Hoechst 33342*CAS 23491-52-3*/17530/100毫克
AAT生物探索/Hoechst 33342*CAS 23491-52-3*/17530/100毫克
AAT生物探索/Hoechst 33342*CAS 23491-52-3*/17530/100毫克
商品编号: 17530
品牌: aatbio
市场价: ¥1900.00
美元价: 1140.00
产地: 美国(厂家直采)
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产品分类: 其他生物染料
公司分类: Other_biological_dyes
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Ex/Em(nm)350/461
MW561.93
CAS#23491-52-3
SolventWater
StorageF/D/L
CategoryNucleicAcidDetection
DNADetection
RelatedLabelingCells
FluorescenceImaging
BiochemicalAssays
TheHoechststainsareafamilyoffluorescentstainsforlabelingDNAinfluorescencemicroscopy.BecausethesefluorescentstainslabelDNA,theyarealsocommonlyusedtovisualizenucleiandmitochondria.Twoofthesecloselyrelatedbis-benzimidesarecommonlyused:Hoechst33258andHoechst33342.Bothdyesareexcitedbyultravioletlightataround350nm,andbothemitblue/cyanfluorescencelightaroundanemissionmaximumat461nm.TheHoechststainsmaybeusedonliveorfixedcells,andareoftenusedasasubstituteforanothernucleicacidstain,DAPI.ThekeydifferencebetweenthemisthattheadditionalethylgroupofHoechst33342rendersitmorelipophilic,andthusmoreabletocrossintactcellmembranes.Insomeapplications,Hoechst33258issignificantlylesspermeant.ThesedyescanalsobeusedtodetectthecontentsofasampleDNAbyplottingastandardemission-to-contentcurve.
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Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

Thefollowingprocedurecanbeadaptedformostcelltypes.Growthmedium,celldensity,thepresenceofothercelltypesandotherfactorsmayinfluencestaining.Residualdetergentonglasswaremayalsoaffectrealorapparentstainingofmanyorganisms,causingbrightlystainedmaterialtoappearinsolutionswithorwithoutcellspresent.

PelletcellsbycentrifugationandresUSPendthecellsinbufferedsaltsolutionsormedia,withoptimaldyebindingatpH7.4.Adherentcellsinculturemaybestainedinsituoncoverslipsorinthecellculturewells.AddHoechststainusingtheconcentrationsbetween0.5and5µMandincubateitfor15to60minutesasaguide.Ininitialexperiments,itmaybebesttotryseveraldyeconcentrationsovertheentiresuggestedrangetodeterminetheconcentrationthatyieldsoptimalstaining.

References&Citations
CitationExplorer

GraftingofRing-OpenedCyclopropylaminethinfilmsonSilicon(100)HydrideviaUVPhotoionization
Authors:JolineTung,JingYuanChing,YokeMooiNg,LihShinTew,YitLungKhung
Journal:ACSAppliedMaterials&Interfaces(2017)

MDA5InducesaStrongerInterferonResponsethanRIG-ItoGCRVInfectionthroughaMechanismInvolvingthePhosphorylationandDimerizationofIRF3andIRF7inCIKCells
Authors:QuanyuanWan,ChunrongYang,YouliangRao,ZhiweiLiao,JianguoSu
Journal:FrontiersinImmunology(2017)

Dual-functionalizedgrapheneoxideforenhancedsiRNAdeliverytobreastcancercells
Authors:RanaImani,WeiShao,SamiraTaherkhani,ShahriarHojjatiEmami,SatyaPrakash,ShahabFaghihi
Journal:ColloidsandSurfacesB:Biointerfaces(2016):315--325

AC-terminalacidicdomainregulatesdegradationofthetranscriptionalcoactivatorBob1
Authors:JohnMLindner,ChristinaSFWong,AndreasMöller,PeterJNielsen
Journal:MolecularandcellularBIOLOGy(2013):4628--4640


品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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