Overview | PrinterFriendlyVersion |
Ex/Em(nm) | 350/461 |
MW | 561.93 |
CAS# | 23491-52-3 |
Solvent | Water |
Storage | F/D/L |
Category | NucleicAcidDetection DNADetection |
Related | LabelingCells FluorescenceImaging BiochemicalAssays |
Spectrum | AdvancedSpectrumViewer |
Thefollowingprocedurecanbeadaptedformostcelltypes.Growthmedium,celldensity,thepresenceofothercelltypesandotherfactorsmayinfluencestaining.Residualdetergentonglasswaremayalsoaffectrealorapparentstainingofmanyorganisms,causingbrightlystainedmaterialtoappearinsolutionswithorwithoutcellspresent.
PelletcellsbycentrifugationandresUSPendthecellsinbufferedsaltsolutionsormedia,withoptimaldyebindingatpH7.4.Adherentcellsinculturemaybestainedinsituoncoverslipsorinthecellculturewells.AddHoechststainusingtheconcentrationsbetween0.5and5µMandincubateitfor15to60minutesasaguide.Ininitialexperiments,itmaybebesttotryseveraldyeconcentrationsovertheentiresuggestedrangetodeterminetheconcentrationthatyieldsoptimalstaining.
References&Citations | CitationExplorer |
GraftingofRing-OpenedCyclopropylaminethinfilmsonSilicon(100)HydrideviaUVPhotoionization
Authors:JolineTung,JingYuanChing,YokeMooiNg,LihShinTew,YitLungKhung
Journal:ACSAppliedMaterials&Interfaces(2017)
MDA5InducesaStrongerInterferonResponsethanRIG-ItoGCRVInfectionthroughaMechanismInvolvingthePhosphorylationandDimerizationofIRF3andIRF7inCIKCells
Authors:QuanyuanWan,ChunrongYang,YouliangRao,ZhiweiLiao,JianguoSu
Journal:FrontiersinImmunology(2017)
Dual-functionalizedgrapheneoxideforenhancedsiRNAdeliverytobreastcancercells
Authors:RanaImani,WeiShao,SamiraTaherkhani,ShahriarHojjatiEmami,SatyaPrakash,ShahabFaghihi
Journal:ColloidsandSurfacesB:Biointerfaces(2016):315--325
AC-terminalacidicdomainregulatesdegradationofthetranscriptionalcoactivatorBob1
Authors:JohnMLindner,ChristinaSFWong,AndreasMöller,PeterJNielsen
Journal:MolecularandcellularBIOLOGy(2013):4628--4640