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AAT Bioquest/Nuclear Yellow [Hoechst S769121] *CAS 74681-68-8*/17539/25 mg
![AAT Bioquest/Nuclear Yellow [Hoechst S769121] *CAS 74681-68-8*/17539/25 mg](https://www.ebiomall.cn/images/AAT201712/chemical-structure-for-nuclear-yellow-hoechst-s769121-cas-74681-68-8.png)
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 355/495 |
| MW | 651.01 |
| CAS# | 74681-68-8 |
| Solvent | DMSO |
| Storage | F/D/L |
| Category | NucleicAcidDetection DNADetection |
| Related | LabelingCells FluorescenceImaging BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
Thefollowingprocedurecanbeadaptedformostcelltypes.Growthmedium,celldensity,thepresenceofothercelltypesandotherfactorsmayinfluencestaining.Residualdetergentonglasswaremayalsoaffectrealorapparentstainingofmanyorganisms,causingbrightlystainedmaterialtoappearinsolutionswithorwithoutcellspresent.
1) Pelletcellsbycentrifugation
2) ResUSPendthecellsinbuffered solutionsormediawithoptimaldyebindingatpH7.4.
3) Stainadherentcellsinsituoncoverslipsorinthecellculturewells.
4) AddHoechststainusingtheconcentrationsbetween0.5and5µM.
5) Incubatethecellsfor15to60minutes.
Note:Theincubationtimeanddyeconcentrationneedexperimentallytested.Ininitialexperiments,itmaybebesttotryseveraldyeconcentrationsovertheentiresuggestedrangetodeterminetheconcentrationthatyieldsoptimalstaining.
| References&Citations |  PrinterFriendlyVersion |
1. Pinna-SennE,LisantiJA,OrtizMI,DalmassoG,BellaJL,GosalvezJ,StockertJC.(2000)Specificheterochromaticbandingofmetaphasechromosomesusingnuclearyellow.BiotechHistochem,75,132.
2. Pinna-SennE,LisantiJA,OrtizMI,DalmassoG,BellaJL,GosalvezJ,StockertJC.(2000)Specificheterochromaticbandingofmetaphasechromosomesusingnuclearyellow.BiotechHistochem,75,132.
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6. StanzaniS,RussoA,LeanzaG.(1984)[Projectionstothesupraopticandparaventricularnucleistudiedintheratbymeansofretrogradefluorescenttracers(fastblue,nuclearyellow)].BollSocItalBiolSper,60,2339.
7. CurtisSK,CowdenRR.(1983)EvaluationoffivebasicFluorochromesofpotentialuseinmicrofluorometricstudiesofnucleicacids.Histochemistry,78,503.
8. PollinB,LaplanteS,CesaroP,Nguyen-LegrosJ.(1983)SimultaneousvisualizationofNuclearyellowandiron-dextrancomplexfordemonstrationofbranchedneuronsbyretrogradeaxonaltransport.JNeurosciMethods,8,205.
9. ArbuthnottGW,WrightAK,HamiltonMH,BrownJR.(1982)OrthogradetransportofNuclearyellow:aproblemanditssolution.JNeurosciMethods,6,365.
10. KatanS,GottschallJ,NeuhuberW.(1982)SimultaneousvisualizationofhorseradishperoxidaseandNuclearYellowintissuesectionsforneuronaldoublelabeling.NeurosciLett,28,121.
