| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 350/461 |
| MW | N/A |
| CAS# | N/A |
| Solvent | DMSO |
| Storage | F/D/L |
| Category | NucleicAcidDetection DNADetection |
| Related | LabelingCells FluorescenceImaging BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
1. AddNuclearBlue™DCS1(2to10µM)intothefixed,deadorapoptoticcells(eithersUSPensionoradherent)andstainthecellsfor15to60minutes.Ininitialexperiments,itmaybebesttotryseveraldyeconcentrationstodeterminetheoptimalconcentrationthatyieldsthedesiredresult.
2. Directlyanalyzethecellularstainingwithfluorescencemicroscopy,fluorescencemicroplatereader,orflowcytometry.Optionaloperation:CarefullywashthecellswithPBSbeforeanalysis.
A
B
Figure1.A:ExcitationandemissionspectrafortheNuclearBlue™DCS1boundtoDNAinTEBuffer.B:DeadcellimagingwithNuclearBlue™DCS1.FixedHeLacellsplatedon96-wellplates,incubatedwithNuclearBlue™DCS12.5µMfor20minutesandimagedwithDAPIchannel.
| References&Citations |  CitationExplorer |
One-StepSeedingofNeuralStemCellswithVitronectin-SupplementedMediumforHigh-ThroughputScreeningAssays
Authors:ShengDai,RongLi,YanLong,SteveTitus,JinghuaZhao,RuiliHuang,MenghangXia,WeiZheng
Journal:JournalofBiomolecularScreening(2016):1112--1124
GenomeshufflingofthenonconventionalyeastPichiaanomalaforimprovedsugaralcoholproduction
Authors:GuoqiangZhang,YupingLin,XianniQi,LixianWang,PengHe,QinhongWang,YanheMa
Journal:Microbialcellfactories(2015):1
