| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 543/712 |
| MW | 471.98 |
| CAS# | 181885-68-7 |
| Solvent | DMSO |
| Storage | F/D/L |
| Category | NucleicAcidDetection DNADetection |
| Related | LabelingCells FluorescenceImaging BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
1. Makea5-10mMDMSOstocksolution.
2. Add1to10µMintothecells(eithersUSPensionoradherentcells),andstainthecellsfor15to60minutes.Ininitialexperiments,itmaybebesttotryseveraldyeconcentrationstodeterminetheoptimalconcentrationthatyieldsthedesiredresult.Highdyeconcentrationtendstocausenonspecificstainingofothercellularstructures.
3. Directlyanalyzethecellularstainingwithafluorescencemicroscope,afluorescencemicroplatereader,orflowcytometer.
| References&Citations |  PrinterFriendlyVersion |
1. KubotaT,IkedaS,YanagisawaH,YukiM,OkamotoA.(2009)Hybridization-sensitivefluorescentprobeforlong-termmonitoringofintracellularRNA.BioconjugChem,20,1256.
2. LugoMR,SharomFJ.(2009)InteractionofLDS-751withthedrug-bindingsiteofP-glycoprotein:aTrpfluorescencesteady-stateandlifetimestudy.ArchBiochemBiophys,492,17.
3. OkamotoA,IkedaS,KubotaT,YukiM,YanagisawaH.(2009)Exciton-controlledfluorescence:applicationtohybridization-sensitivefluorescentDNAprobe.NucleicAcidsSympSer(Oxf),49.
4. WiczlingP,KrzyzanskiW.(2008)Flowcytometricassessmentofhomeostaticagingofreticulocytesinrats.ExpHematol,36,119.
5. GuoX,WangS,XiaA,SuH.(2007)Determinationoftheformationofdarkstateviadepletedspontaneousemissioninacomplexsolvatedmolecule.JPhysChemA,111,5800.
6. MaesML,DavidsonLB,McDonaghPF,RitterLS.(2007)ComparisonofsamplefixationandtheuseofLDS-751oranti-CD45forleukocyteidentificationinmousewholebloodforflowcytometry.JImmunolMethods,319,79.
7. FritschM,StarrussJ,LoescheA,MuellerS,BleyT.(2005)CellcyclesynchronizationofCupriavidusnecatorbycontinuousphasingmeasuredviaflowcytometry.BiotechnolBioeng,92,635.
8. LugoMR,SharomFJ.(2005)InteractionofLDS-751andrhodamine123withP-glycoprotein:evidenceforsimultaneousbindingofbothdrugs.Biochemistry,44,14020.
9. LugoMR,SharomFJ.(2005)InteractionofLDS-751withP-glycoproteinandmappingofthelocationoftheRdrugbindingsite.Biochemistry,44,643.
10. SetzPD,KnochenmussR.(2005)ExcitonmobilityandtrappinginaMALDImatrix.JPhysChemA,109,4030.
