>
产品中心 >
Other_biological_dyes >
AAT Bioquest/Cyber Green™ Nucleic Acid Gel Stain [Equivalent to SYBR® Green] *10,000X DMSO Solution*/17590/1 ml
![AAT Bioquest/Cyber Green™ Nucleic Acid Gel Stain [Equivalent to SYBR® Green] *10,000X DMSO Solution*/17590/1 ml](https://www.ebiomall.cn/images/AAT201712/hq-figure-1-for-cyber-green-nucleic-acid-gel-stain-sybr-green-10-000x-dmso-solution.png)
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 497/520 |
| MW | N/A |
| CAS# | N/A |
| Solvent | N/A |
| Storage | F/D/L |
| Category | NucleicAcidDetection DNADetection |
| Related | LabelingCells FluorescenceImaging BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
1.Post-stainingProtocol
1.1Rungelsbasedonyourstandardprotocol.
1.2Make1XCyberGreen™workingsolutionbydilutingthe10,000XstockreagentintoPH7.5-8buffer
(e.g.,TAE,TBEorTEpreferablypH8.0).
Note:Stainingsolutionspreparedinwaterarelessstablethanthosepreparedinbufferandmustbeusedwithin24hourstoensuremaximalstainingsensitivity.Inaddition,stainingsolutionspreparedinbufferswithpHbelowabout7.5orabove8.0arelessstableandshowreducedstainingefficacy.
1.3Placethegelinasuitablepolypropylenecontainer.Gentlyaddasufficientamountofthe1Xstaining
solutiontosubmergethegel.
Note:Donotuseaglasscontainer,asitwilladsorbmuchofthedyeinthestainingsolution.
1.4Agitatethegelgentlyatroomtemperaturefor~30minutes,protectingfromthelight.
Note:Thestainingsolutionmaybestoredinthedark(preferablyrefrigerated)foraweekandreusedupto2-3times.
1.5Imagethestainedgelwitha254nmtransilluminator,oralaser-basedgelscannerusingalongpathgreenfiltersuchasaSYBR®filterorGelStar®filter.
2.Pre-castingprotocol
2.1Prepareagarosegelsolutionusingyourstandardprotocol.
2.2Dilutethe10,000XCyberGreen™stockreagentintothegelsolutionat1:10,000justpriortopouringthe
gelandmixthoroughly.
2.3Rungelsbasedonyourstandardprotocol.
2.4Imagethestainedgelwitha254nmtransilluminator,oralaser-basedgelscannerusingalongpathgreenfiltersuchasaSYBR®filterorGelStar®filter.
3.DNA-stainingbeforeelectrophoresis
3.1IncubateDNAwitha1:10,000dilutionofthedye(inTE,TBE,orTAE)foratleast15minutespriortoelectrophoresis.
3.2Rungelsbasedonyourstandardprotocol.
3.3Imagethestainedgelwitha254nmtransilluminator,oralaser-basedgelscannerusingalongpathgreenfiltersuchasaSYBR®filterorGelStar®filter.
| References&Citations |  PrinterFriendlyVersion |
1. ZhuX,TaoL,Tejima-MandevilleE,QiuJ,ParkJ,GarberK,EricssonM,LoEH,WhalenMJ.(2012)PlasmalemmapermeABIlityandnecroticcelldeathphenotypesafterintracerebralhemorrhageinmice.Stroke,43,524.
2. ParkJ,EstradaA,SchwartzJA,DiagaradjaneP,KrishnanS,DunnAK,TunnellJW.(2010)Intra-organBiodistributionofGoldNanoparticlesUsingIntrinsicTwo-photonInducedPhotoluminescence.LasersSurgMed,42,630.
3. van"tHoffM,ReuterM,DrydenDT,OheimM.(2009)Screeningbyimaging:scalingupsingle-DNA-moleculeanalysiswithanovelparabolicVA-TIRFreflectorandnoise-reductiontechniques.PhysChemChemPhys,11,7713.
4. LeeBC,KimS,SohKS.(2008)NovelanatomicstructuresinthebrainandspinalcordofrabbitthatmaybelongtotheBonghansystemofpotentialacupuncturemeridians.JAcupunctMeridianStud,1,29.
5. ShimizuM,SasakiS,KinjoM.(2007)TripletfractionbuildupeffectoftheDNA-YOYOcomplexstudiedwithfluorescencecorrelationspectroscopy.AnalBiochem,366,87.
6. PloegerLS,HuismanA,vanderGugtenJ,vanderGiezenDM,BelienJA,AbbakerAY,DullensHF,GrizzleW,PoulinNM,MeijerGA,vanDiestPJ.(2005)ImplementationofaccurateandfastDNAcytometrybyconfocalmicroscopyin3D.CellOncol,27,225.
7. ShimizuM,SasakiS,TsuruokaM.(2005)DNAlengthevaluationusingcyaninedyeandfluorescencecorrelationspectroscopy.Biomacromolecules,6,2703.
8. ZhangWH,ZhuSN,LuSL,HuangYL,ZhaoP.(2000)Three-dimensionalimageofhepatocellularcarcinomaunderconfocallaserscanningmicroscope.WorldJGastroenterol,6,344.
9. CosaG,FocsaneanuKS,McLeanJR,McNameeJP,ScaianoJC.(2001)PhotophysicalpropertiesoffluorescentDNA-dyesboundtosingle-anddouble-strandedDNAinaqueousbufferedsolution.PhotochemPhotobiol,73,585.
10. WongM,KongS,DragowskaWH,BallyMB.(2001)OxazoleyellowhomodimerYOYO-1-labeledDNA:afluorescentcomplexthatcanbeusedtoassessstructuralchangesinDNAfollowingformationandcellulardeliveryofcationiclipidDNAcomplexes.BiochimBiophysActa,1527,61.
