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AAT Bioquest/赛博绿色与贸易[相当于SYBR®；绿色]*10000x PCR水溶液*/17592/1毫升
![AAT Bioquest/赛博绿色与贸易[相当于SYBR®；绿色]*10000x PCR水溶液*/17592/1毫升](https://www.ebiomall.cn/images/AAT201712/hq-figure-1-for-cyber-green-sybr-green-10-000x-aqueous-pcr-solution.png)
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 497/520 |
| MW | N/A |
| CAS# | N/A |
| Solvent | N/A |
| Storage | F/D/L |
| Category | NucleicAcidDetection DNADetection |
| Related | LabelingCells FluorescenceImaging |
| Spectrum | AdvancedSpectrumViewer |
- Make20XCyberGreen™solutionbyadding2uLof10,000XCyberGreen™DMSOsolutionto1mLddH2O.
- SetupthePCRreactionasfollows:
5μLof10xpolymerasebufferwithoutMg2+
2.5µLof50mMMgCl2
5µLeachof2mMdNTP
2.5µLof20XCyberGreen™(fromStep1)
1-5unitsofTaqDNApolymerase
0.1-1µMeachofprimers(finalconcentrations)
ddH2Otoafinalvolumeof50µL. - Performreal-timePCRonaThermocyclingfluorimeterandrecordthefluorescencesignalattheannealingorextensionstep.
Note:WhenusingABISequenceDetectionSystems,makesuretoselectNONEforthepassivereferenceunderthetabWELLINSPECTOR.
| References&Citations |  PrinterFriendlyVersion |
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3. van"tHoffM,ReuterM,DrydenDT,OheimM.(2009)Screeningbyimaging:scalingupsingle-DNA-moleculeanalysiswithanovelparabolicVA-TIRFreflectorandnoise-reductiontechniques.PhysChemChemPhys,11,7713.
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7. ShimizuM,SasakiS,TsuruokaM.(2005)DNAlengthevaluationusingcyaninedyeandfluorescencecorrelationspectroscopy.Biomacromolecules,6,2703.
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9. CosaG,FocsaneanuKS,McLeanJR,McNameeJP,ScaianoJC.(2001)PhotophysicalpropertiesoffluorescentDNA-dyesboundtosingle-anddouble-strandedDNAinaqueousbufferedsolution.PhotochemPhotobiol,73,585.
10. WongM,KongS,DragowskaWH,BallyMB.(2001)OxazoleyellowhomodimerYOYO-1-labeledDNA:afluorescentcomplexthatcanbeusedtoassessstructuralchangesinDNAfollowingformationandcellulardeliveryofcationiclipidDNAcomplexes.BiochimBiophysActa,1527,61.
