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AAT Bioquest/Cyber Green™ [Equivalent to SYBR® Green] *20X Aqueous PCR Solution*/17591/5x1 ml
![AAT Bioquest/Cyber Green™ [Equivalent to SYBR® Green] *20X Aqueous PCR Solution*/17591/5x1 ml](https://www.ebiomall.cn/images/AAT201712/hq-figure-1-for-cyber-green-sybr-green-20x-aqueous-pcr-solution.png)
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 497/520 |
| MW | N/A |
| CAS# | N/A |
| Solvent | N/A |
| Storage | F/D/L |
| Category | NucleicAcidDetection DNADetection |
| Related | LabelingCells FluorescenceImaging BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
1.SetupthePCRreactionasfollows:
5μLof10xpolymerasebufferwithoutMg+2
2.5μLof50mMMgCl2
5μLeachof2mMdNTP
2.5μLof20XCyberGreen™
1-5unitsofTaqDNApolymerase
0.1-1μMeachofprimers(finalconcentrations)
ddH2Otoafinalvolumeof50μL.
2.Performreal-timePCRonaThermocyclingfluorometerandrecordthefluorescencesignalattheannealingorextensionstep.
Note:WhenusingABISequenceDetectionSystems,makesuretoselectNONEforthepassivereferenceunderthetabWELLINSPECTOR.
| References&Citations |  PrinterFriendlyVersion |
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10. WongM,KongS,DragowskaWH,BallyMB.(2001)OxazoleyellowhomodimerYOYO-1-labeledDNA:afluorescentcomplexthatcanbeusedtoassessstructuralchangesinDNAfollowingformationandcellulardeliveryofcationiclipidDNAcomplexes.BiochimBiophysActa,1527,61.
