| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 495/540 |
| MW | N/A |
| CAS# | N/A |
| Solvent | N/A |
| Storage | F/D/L |
| Category | NucleicAcidDetection DNADetection |
| Related | LabelingCells FluorescenceImaging BiochemicalAssays |
1.PreparingGelite™Orangeworkingsolution
1.1 PrepareGelite™Orangeworkingsolutionbyadding1µLofGelite™Orange(ComponentA)into200µLof5XGelLoADIngBuffer(ComponentB). Protecttheworkingsolutionfromlightbycoveringitwithfoilorplacingitinthedark.
2.PrepareDNAloadingsamples
2.1 PrepareDNAsamplesasyoudesired.
2.2 Add4µLofGelite™Orangeworkingsolution(fromStep1.1)into16µLofDNAsamples(fromStep2.1),mixwell.Incubateatroomtemperaturefor5-15minutespriortoelectrophoresis.
2.3 Rungelsbasedonyourstandardprotocol.
2.4 Imagethegelwitha300nmultravioletor254nmtransilluminator,oralaser-basedgelscannerusingalongpathgreenfiltersuchasaSYBR®filterorGelStar®filter.
| References&Citations |  PrinterFriendlyVersion |
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