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AAT Bioquest/Cyber Orange™ Nucleic Acid Gel Stain *10,000X DMSO Solution*/17595/1 ml
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 495/540 |
| MW | N/A |
| CAS# | N/A |
| Solvent | N/A |
| Storage | F/D/L |
| Category | NucleicAcidDetection DNADetection |
| Related | LabelingCells FluorescenceImaging BiochemicalAssays |
1.Post-stainingProtocol
1.1Rungelsbasedonyourstandardprotocol.
1.2Make1XCyberOrange™workingsolutionbydilutingthe10,000XstockreagentintoPH7.5-8buffer (e.g.,TAE,TBEorTEpreferablypH8.0).
Note:Stainingsolutionspreparedinwaterarelessstablethanthosepreparedinbufferandmustbeusedwithin24hourstoensuremaximalstainingsensitivity.Inaddition,stainingsolutionspreparedinbufferswithpHbelowabout7.5orabove8.0arelessstableandshowreducedstainingefficacy.
1.3Placethegelinasuitablepolypropylenecontainer.Gentlyaddasufficientamountofthe1Xstainingsolutionto submergethegel.
Note:Donotuseaglasscontainer,asitwilladsorbmuchofthedyeinthestainingsolution.
1.4Agitatethegelgentlyatroomtemperaturefor~30minutes,protectingfromthelight.
Note:Thestainingsolutionmaybestoredinthedark(preferablyrefrigerated)foraweekandreusedupto2-3times.
1.5Imagethegelwitha300nmultravioletor254nmtransIlluminator,oralaser-basedgelscannerusingalongpathgreenfiltersuchasaSYBR®filterorGelStar®filter.
2.Pre-castingprotocol
2.1Prepareagarosegelsolutionusingyourstandardprotocol.
2.2Dilutethe10,000XCyberOrange™stockreagentintothegelsolutionat
1:10,000justpriortopouringthe gelandmixthoroughly.
2.3Rungelsbasedonyourstandardprotocol.
2.4Imagethegelwitha300nmultravioletor254nmtransilluminator,oralaser-based
gelscannerusingalongpathgreenfiltersuchasaSYBR®filterorGelStar®filter.
| References&Citations |  PrinterFriendlyVersion |
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8. LiF,KanB,WangD.(2014)[DevelopmentofbothmultiplePCRandreal-timeSYBRgreenPCRforthedetectionofVibriocholeraenon-O1/O139SEROgroups].ZhonghuaLiuXingBingXueZaZhi,35,66.
9. AnthonyJohnsonAM,DasguptaI,SaiGopalDV.(2014)Developmentofloop-mediatedisothermalamplificationandSYBRgreenreal-timePCRmethodsforthedetectionofCitrusyellowmosaicbadnavirusincitrusspecies.JVirolMethods,203,9.
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