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AAT Bioquest/FluoroQuest™ Anti-fading Kit I *Optimized for Slide Imaging*/20001/1 kit
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | None/None |
| MW | N/A |
| CAS# | N/A |
| Solvent | N/A |
| Storage | F/D/L |
| Category | MicroBIOLOGy FluorescenceImaging |
| Related | BiochemicalAssays |
1.Thawthecomponents:Thawallthekitcomponentsatroomtemperature,andkeepfromlight.
2.Applyanti-fadingreagent:Removeanyexcessliquidfromyourspecimen.Addasmalldropoftheselectedcomponenttothespecimen.Ifthesampleisonaslideortissueculturedish,carefullyplaceacoversliponthedrop,avoidingairbubbles.Ifthesampleisonacoverslip,invertthecoversliponacleanglassslide.Removeanyexcessanti-fadingcomponent.
3.Preparesamplesforimaging:Theanti-fadingreagentsshouldbeincubatedfor2hourstoovernight.Forlong-termstorage,sealthecoversliptotheslidewithnailpolishoraplasticsealant.Mountedslidesshouldbestoredat4ºCinthedarkforoptimumsamplelongevity.Thefluorescenceimagingwouldremainstableformanyweeks.Samplescanbeimagedimmediatelyaftermounting.AtypicalimageisshowninFigure1.
Note:Theseready-to-useanti-fadingreagentscanbeapplieddirectlyonthewashedspecimen.Althoughthereagentshavebeentestedwithlotsoffixedsamples,theiroptimalanti-fadingefficienciesstronglydependonthepropertiesofyoursamples.Wesuggestthatyoutrymorethanonecomponentforyourimagingsamplestogettheidealcomponent.Forexample,onecomponentmaybemorecompatIBLewithafluorescentlabeledantibodyconjugate(oranenzymesubstrateoraspecialmountingspecimensthatcontainlipophilicplasmamembranestainslikeDiI)thananotherone.
| References&Citations |  PrinterFriendlyVersion |
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