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AAT Bioquest/FluoroQuest™ Mounting Medium with DAPI/20004/50 mL
Overview | PrinterFriendlyVersion |
Ex/Em(nm) | 358/461 |
MW | N/A |
CAS# | N/A |
Solvent | Water |
Storage | F/D/L |
Category | MicroBIOLOGy FluorescenceImaging |
Related | BiochemicalAssays |
Spectrum | AdvancedSpectrumViewer |
1.Warmupthemountingsolution:Removethemountingsolutionfromrefrigerator.Warmthebottletoroomtemperature,protectedfromlight.
2.Applythemountingsolution:Removeanyexcessliquidfromyourspecimen.Addasmalldropofthemountingsolutiontothespecimen.Ifthesampleisonaslideortissueculturedish,carefullyplaceacoversliponthedrop,avoidingairbubbles.Ifthesampleisonacoverslip,invertthecoversliponacleanglassslide.Removeanyexcessanti-fADIngcomponent.
3.Preparesamplesforimaging:Themountingsolutionshouldbeincubatedfor2hourstoovernight.Forlong-termstorage,sealthecoversliptotheslidewithnailpolishoraplasticsealant.Mountedslidesshouldbestoredat4ºCinthedarkforoptimumsamplelongevity.Thefluorescenceimagingwouldremainstableformanyweeks.Samplescanbeimagedimmediatelyaftermounting.
References&Citations | PrinterFriendlyVersion |
1. CordesT,VogelsangJ,TinnefeldP.(2009)OnthemechanismofTroloxasantiblinkingandantibleachingreagent.JAmChemSoc,131,5018.
2. Gallardo-EscarateC,Alvarez-BorregoJ,VonBrandE,DupreE,DelRio-PortillaMA.(2007)RelationshipbetweenDAPI-fluorescencefadingandnuclearDNAcontent:AnalternativemethodtoDNAquantification?BiolRes,40,29.
3. WidengrenJ,ChmyrovA,EggelingC,LofdahlPA,SeidelCA.(2007)StrategiestoimprovephotostABIlitiesinultrasensitivefluorescencespectroscopy.JPhysChemA,111,429.
4. DikiciogluE,MeteogluI,OkyayP,CulhaciN,KacarF.(2003)Thereliabilityoflong-termstorageofdirectimmunofluorescentstainingslidesatroomtemperature.JCutanPathol,30,430.
5. El-RifaiWE,KnuutilaS.(2001)Comparativegenomichybridizationtechnique.MethodsMolMed,50,25.
6. BerriosM,ConlonKA,ColfleshDE.(1999)Antifadingagentsforconfocalfluorescencemicroscopy.MethodsEnzymol,307,55.
7. BrockR,HamelersIH,JovinTM.(1999)ComparisonoffixationprotocolsforadherentculturedcellsappliedtoaGFPfusionproteinoftheepidermalgrowthfactorreceptor.Cytometry,35,353.
8. KolankoCJ,PyleMD,LoatsH,PartonJ,BlakelyWF,NathJ.(1999)Fast-insituhybridizationandimmunoenzymaticcolorpigmentdetectionofmousebonemarrowmicronucleus.BiotechHistochem,74,111.
9. BenchaibM,DelormeR,PluvinageM,BryonPA,SouchierC.(1996)Evaluationoffivegreenfluorescence-emittingstreptavidin-conjugatedFluorochromesforuseinimmunofluorescencemicroscopy.HistochemCellBiol,106,253.
10. SwartzDJ,SantiPA.(1996)ImmunofluorescentartifactsduetothepHofantifadingmountingmedia.Biotechniques,20,398.