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主营:主营:研究并生产荧光和发光探针,信号转导研究的试剂
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当前位置: 首页 > 产品中心 > Fluorescent_dyes > AAT Bioquest/FluoroQuest™ Mounting Medium with DAPI/20004/50 mL
商品详细AAT Bioquest/FluoroQuest™ Mounting Medium with DAPI/20004/50 mL
AAT Bioquest/FluoroQuest™ Mounting Medium with DAPI/20004/50 mL
AAT Bioquest/FluoroQuest™ Mounting Medium with DAPI/20004/50 mL
商品编号: 20004
品牌: aatbio
市场价: ¥27560.00
美元价: 16536.00
产地: 美国(厂家直采)
公司:
产品分类: 荧光染料
公司分类: Fluorescent_dyes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Overview
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Ex/Em(nm)358/461
MWN/A
CAS#N/A
SolventWater
StorageF/D/L
CategoryMicroBIOLOGy
FluorescenceImaging
RelatedBiochemicalAssays
FluoroQuest™withDAPIisanaqueousmountingmediumforpreservingfluorescenceoftissueandcellsmears.ThismountingmediumisfortifiedwithDAPIwhichisacounterstainforDNA.ForuseininsituhybridizationtechniquesorothermethodswherefluorescenceofDNAstainingisrequired.DAPIexcitesat360nmandemitsat460nm,producingabluefluorescence.RNAisalsostainedwithDAPI.
SpectrumAdvancedSpectrumViewer

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Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.Warmupthemountingsolution:Removethemountingsolutionfromrefrigerator.Warmthebottletoroomtemperature,protectedfromlight.

2.Applythemountingsolution:Removeanyexcessliquidfromyourspecimen.Addasmalldropofthemountingsolutiontothespecimen.Ifthesampleisonaslideortissueculturedish,carefullyplaceacoversliponthedrop,avoidingairbubbles.Ifthesampleisonacoverslip,invertthecoversliponacleanglassslide.Removeanyexcessanti-fADIngcomponent.

3.Preparesamplesforimaging:Themountingsolutionshouldbeincubatedfor2hourstoovernight.Forlong-termstorage,sealthecoversliptotheslidewithnailpolishoraplasticsealant.Mountedslidesshouldbestoredat4ºCinthedarkforoptimumsamplelongevity.Thefluorescenceimagingwouldremainstableformanyweeks.Samplescanbeimagedimmediatelyaftermounting.

References&Citations
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1.   CordesT,VogelsangJ,TinnefeldP.(2009)OnthemechanismofTroloxasantiblinkingandantibleachingreagent.JAmChemSoc,131,5018.

2.   Gallardo-EscarateC,Alvarez-BorregoJ,VonBrandE,DupreE,DelRio-PortillaMA.(2007)RelationshipbetweenDAPI-fluorescencefadingandnuclearDNAcontent:AnalternativemethodtoDNAquantification?BiolRes,40,29.

3.   WidengrenJ,ChmyrovA,EggelingC,LofdahlPA,SeidelCA.(2007)StrategiestoimprovephotostABIlitiesinultrasensitivefluorescencespectroscopy.JPhysChemA,111,429.

4.   DikiciogluE,MeteogluI,OkyayP,CulhaciN,KacarF.(2003)Thereliabilityoflong-termstorageofdirectimmunofluorescentstainingslidesatroomtemperature.JCutanPathol,30,430.

5.   El-RifaiWE,KnuutilaS.(2001)Comparativegenomichybridizationtechnique.MethodsMolMed,50,25.

6.   BerriosM,ConlonKA,ColfleshDE.(1999)Antifadingagentsforconfocalfluorescencemicroscopy.MethodsEnzymol,307,55.

7.   BrockR,HamelersIH,JovinTM.(1999)ComparisonoffixationprotocolsforadherentculturedcellsappliedtoaGFPfusionproteinoftheepidermalgrowthfactorreceptor.Cytometry,35,353.

8.   KolankoCJ,PyleMD,LoatsH,PartonJ,BlakelyWF,NathJ.(1999)Fast-insituhybridizationandimmunoenzymaticcolorpigmentdetectionofmousebonemarrowmicronucleus.BiotechHistochem,74,111.

9.   BenchaibM,DelormeR,PluvinageM,BryonPA,SouchierC.(1996)Evaluationoffivegreenfluorescence-emittingstreptavidin-conjugatedFluorochromesforuseinimmunofluorescencemicroscopy.HistochemCellBiol,106,253.

10.   SwartzDJ,SantiPA.(1996)ImmunofluorescentartifactsduetothepHofantifadingmountingmedia.Biotechniques,20,398.


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品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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