Overview | PrinterFriendlyVersion |
Ex/Em(nm) | 780/800 |
MW | 1016.05 |
CAS# | N/A |
Solvent | DMSO |
Storage | F/D/L |
Category | NeuroBIOLOGy ReactiveOxygenSpecies |
Related | CellSignaling BiochemicalAssays |
Spectrum | AdvancedSpectrumViewer |
1) Preparea5to10mMDAX-J2™stocksolutioninDMSO.Make1to10µMworkingsolutionbydilutingtheDMSOstocksolutionintoHankssolutionwith20mMHepesbuffer(HHBS).
2) Treatcellsasdesired.
3) Incubatethecellswith1-10µM(fromStep#1)DAX-J2™orange(cat#16300),Red(cat#16301) orRatio580/460(cat#16310)for20-60minutesat37ºC.
4) Replacethedye-loADIngsolutionwithHHBSbuffer.
5) Analyzethecellswithaproperfluorescenceinstrumentwithbottomreadmode.
References&Citations | CitationExplorer |
Fluorescentreal-timequantitativemeasurementsofintracellularperoxynitritegenerationandinhibition
Authors:ZhenLuo,QinZhao,JixiangLiu,JinfangLiao,RuoguPeng,YuntingXi,ZhenjunDiwu
Journal:AnalyticalBiochemistry(2017)
InducibleNitricOxideSynthase(iNOS)IsaNovelNegativeRegulatorofHematopoieticStem/ProgenitorCellTrafficking
Authors:MateuszAdamiak,AhmedABDelbaset-Ismail,JosephBMoore,JZhao,AhmedAbdel-Latif,MarcinWysoczynski,MariuszZRatajczak
Journal:StemCellReviewsandReports(2016):1--12