| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 355/430 |
| MW | 461.62 |
| CAS# | 115534-33-3 |
| Solvent | Water |
| Storage | F/D/L |
| Category | IonChannels MembranePotentials |
| Related | NeurologicalStains BiochemicalAssays |
Thefollowingprocedurecanbeadaptedformostcelltypes.Growthmedium,celldensity,thepresenceofothercelltypesandotherfactorsmayinfluencestaining.Residualdetergentonglasswaremayalsoaffectrealorapparentstainingofmanyorganisms,causingbrightlystainedmaterialtoappearinsolutionswithorwithoutcellspresent.
1) PelletcellsbycentrifugationandresUSPendthecellsinbufferedsaltsolutionsormedia,withoptimaldyebindingatpH7.4.Adherentcellsinculturemaybestainedinsituoncoverslipsorinthecellculturewells.
2) MakeaTMA-DPHDMSOstocksolutionat10-50mM.
3) AddTMA-DPHstainusingtheconcentrationsbetween0.5and5µMinHanksand20mMHepesbufferpH7.4,andincubateitat37°C,5%CO2incubatorfor5minutes.Ininitialexperiments,itmaybebesttotryseveraldyeconcentrationsovertheentiresuggestedrangetodeterminetheconcentrationthatyieldsoptimalstaining.
4) WashthecellsandresuspendedintheappropriateHepesbufferpH7.4.
| References&Citations |  CitationExplorer |
CytotoxicityandeffluxpumpinhibitioninducedbymolyBDenumdisulfideandboronnitridenanomaterialswithsheet-likestructure
Authors:SuLiu,ZhuoyanShen,BingWu,YueYu,HuiHou,Xu-XiangZhang,Hong-qiangRen
Journal:EnvironmentalScience&Technology(2017)
InteractionofHsp90withphospholipidmodelmembranes
Authors:MuhanZhang,DaoyingWang,PengpengLi,ChongSun,RongXu,ZhimingGeng,WeiminXu,ZhaoqiDai
Journal:BiochimicaetBiophysicaActa(BBA)-Biomembranes(2017)
ComparisonofCytotoxicityandInhibitionofMembraneABCTransportersInducedbyMWCNTswithDifferentLengthandFunctionalGroups
Authors:JingYu,SuLiu,BingWu,ZhuoyanShen,GaryNCherr,Xu-XiangZhang,MeiLi
Journal:Environmentalscience&technology(2016):3985--3994
Lowlevelsofgrapheneandgrapheneoxideinhibitcellularxenobioticdefensesystemmediatedbyeffluxtransporters
Authors:SuLiu,WeiJiang,BingWu,JingYu,HaiyanYu,Xu-XiangZhang,CristinaTorres-Duarte,GaryNCherr
Journal:Nanotoxicology(2016):597--606
