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当前位置: 首页 > 产品中心 > Other_biological_dyes > AAT Bioquest/Di-4-ANEPPS*CAS#:90134-00-2*/21498/5 mg
商品详细AAT Bioquest/Di-4-ANEPPS*CAS#:90134-00-2*/21498/5 mg
AAT Bioquest/Di-4-ANEPPS*CAS#:90134-00-2*/21498/5 mg
AAT Bioquest/Di-4-ANEPPS*CAS#:90134-00-2*/21498/5 mg
商品编号: 21498
品牌: aatbio
市场价: ¥56560.00
美元价: 33936.00
产地: 美国(厂家直采)
公司:
产品分类: 其他生物染料
公司分类: Other_biological_dyes
联系Q Q: 3392242852
电话号码: 4000-520-616
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商品介绍
Overview
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Ex/Em(nm)497/705
MW480.66
CAS#90134-00-2
SolventDMSO
StorageF/D/L
CategoryIonChannels
MembranePotentials
RelatedNeurologicalStains
BiochemicalAssays
Potential-sensitiveANEPdyesarezwitterionicmolecule,exhibitingthemostconsistentpotentiometricresponseindifferentcellandtissuetypes.Theycanbeintroducedintocellsbydirectadditionofstocksolutiontocellculturemedium,byusingPluronic®F-127,orbyretrogradelabeling.ANEPdyesarenon-fluorescentuntilboundtomembranes,andtheirfluoresceresponsestoelectricalpotentialchangesintheirenvironment.ANEPdyesarefast-responseprobesthatoperatebymeansofachangeintheirelectronicstructure,andconsequentlytheirfluorescenceproperties,inresponsetoachangeinthesurroundingelectricfield.Theiropticalresponseissufficientlyfasttodetecttransient(millisecond)potentialchangesinexcitablecells,includingsingleneurons,cardiaccells,andintactbrains.However,themagnitudeoftheirpotential-dependentfluorescencechangeisoftensmall;fast-responseprobestypicallyshowa2-10%fluorescencechangeper100mV.FurThermore,thesedyesdisplayapotential-dependentshiftintheirexcitationspectra,thuspermittingthequantitationofmembranepotentialusingexcitationratiomeasurements.
SpectrumAdvancedSpectrumViewer

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References&Citations
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1.   LarsenAP,SciutoKJ,MorenoAP,PoelzingS.(2012)Thevoltage-sensitivedyedi-4-ANEPPSslowsconductionvelocityinisolatedguineapighearts.HeartRhythm,9,1493.

2.   BachtelAD,GrayRA,StohlmanJM,BourgeoisEB,PollardAE,RogersJM.(2011)Anovelapproachtodualexcitationratiometricopticalmappingofcardiacactionpotentialswithdi-4-ANEPPSusingpulsedLEDexcitation.IEEETransBiomedEng,58,2120.

3.   OsakaiT,SawadaJ,NagataniH.(2009)Potential-modulatedfluorescencespectroscopyofthemembranepotential-sensitivedyedi-4-ANEPPSatthe1,2-dichloroethane/waterinterface.AnalBioanalChem,395,1055.

4.   HardyME,PollardCE,SmallBG,Bridgland-TaylorM,WoodsAJ,ValentinJP,ABI-GergesN.(2009)Validationofavoltage-sensitivedye(di-4-ANEPPS)-basedmethodforassessingdrug-induceddelayedrepolarisationinbeagledogleftventricularmidmyocardialmyocytes.JPharmacolToxicolMethods,60,94.

5.   NovakovaM,BardonovaJ,ProvaznikI,TaborskaE,BochorakovaH,PaulovaH,HorkyD.(2008)Effectsofvoltagesensitivedyedi-4-ANEPPSonguineapigandrabbitmyocardium.GenPhysiolBiophys,27,45.

6.   XuZH,ZhangZX,WangJ,ZhangH,LiZ,JinYS,DingHY.(2007)[Spectralstudyofvoltagesensitivedyedi-4-ANEPPS].GuangPuXueYuGuangPuFenXi,27,1359.

7.   DumasJH,3rd,KnisleySB.(2005)Two-photonexcitationofdi-4-ANEPPSforopticalrecordingofactionpotentialsinrabbitheart.AnnBiomedEng,33,1802.

8.   TominagaT,TominagaY,YamadaH,MatsumotoG,IchikawaM.(2000)QuantificationofopticalsignalswithelectrophysiologicalsignalsinneuralactivitiesofDi-4-ANEPPSstainedrathippocampalslices.JNeurosciMethods,102,11.

9.   JohnsonPL,SmithW,BaynhamTC,KnisleySB.(1999)ErrorscausedbycombinationofDi-4ANEPPSandFluo3/4forsimultaneousmeasurementsoftransmembranepotentialsandintracellularcalcium.AnnBiomedEng,27,563.

10.   ChaloupkaR,PlasekJ,SlavikJ,SiglerovaV,SiglerK.(1997)MeasurementofmembranepotentialinSaccharomycescerevisiaebytheelectrochromicprobedi-4-ANEPPS:effectofintracellularprobedistribution.FoliaMicrobiol(Praha),42,451.


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品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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