| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 510/524 |
| MW | ~400 |
| CAS# | N/A |
| Solvent | DMSO |
| Storage | F/D/L |
| Category | NeuroBIOLOGy ReactiveOxygenSpecies |
| Related | CellFunctionalAnalysis RedoxEnzymes BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
1.PrepareThioliteGreen™workingsolution:
1.1 Preparea10to25mMstocksolutionofThiolite™Greeninhigh-quality,anhydrousDMSO.Thestocksolutionshouldbeusedpromptly;anyremainingsolutionneedbealiquotedandfrozenat‑20oC.
Note:TheunusedThioliteGreenstocksolutionshouldbedividedintosingleusealiquotsandstoredat-20oC,protectedfromlight.
1.2 Preparea2XThiolite™Greenworkingsolution:Onthedayoftheexperiment,eitherdissolveThiolite™GreeninDMSOorthawanaliquotoftheThiolite™Greenstocksolutiontoroomtemperature.Preparea2Xworkingsolutionatthefinalconcentrationrangingfrom100to250µMin20mMHepesbufferorbufferofyourchoice,pH7.ItisrecommendedtouseThiolite™Greenatthefinalconcentrationrangingfrom50to100µMtomeasureThiolconcentrationinsolution.
2.RunGSHAssayinsupernatants:
2.1 Add50µLof2XThiolite™Greenworkingsolution(fromStep1.2)toeachwelloftheGSHstandard,blankcontrol,andtestsamplestomakethetotalGSHassayvolumeof100µL/well.
Note:Fora384-wellplate,add25µLofsampleand25µLofGSHreactionmixtureintoeachwell.
2.2 Incubatethereactionatroomtemperaturefor10to60minutes,protectedfromlight.
2.3 MonitorthefluorescenceincreasewithafluorescenceplatereaderatEx/Em=490/525nm.
2.4 Thefluorescenceinblankwells(withtheassaybufferonly)isusedasacontrol,andissubtractedfromthevaluesforthosewellswiththethiolreaction.
| References&Citations |  CitationExplorer |
GasChromatography/MassSpectrometry-BasedMetabolomicProfilingRevealsAlterationsinMousePlasmaandLiverinResponsetoFavaBeans
Authors:ManXiao,GuankuiDu,GuobingZhong,DongjingYan,HuazongZeng,WangweiCai
Journal:PloSone(2016):e0151103
Virus-likeparticleswithremovablecyclodextrinsenableglutathione-triggereddrugreleaseincells
Authors:KenichiNiikura,NaotoshiSugimura,YusukeMusashi,ShintaroMikuni,YasutakaMatsuo,ShintaroKobayashi,KeitaNagakawa,ShukoTakahara,ChieTakeuchi,HirofumiSawa
Journal:Molecularbiosystems(2013):501--507
