>
产品中心 >
Other_biological_dyes >
AAT Bioquest/JC-1[5,5,6,6-四氯-1,1,3,3-四乙基苯并咪唑基碳菁碘]*CAS#：3520-43-2*/22201/50 mg
![AAT Bioquest/JC-1[5,5,6,6-四氯-1,1,3,3-四乙基苯并咪唑基碳菁碘]*CAS#：3520-43-2*/22201/50 mg](https://www.ebiomall.cn/images/AAT201712/chemical-structure-for-jc-1-5-5-6-6-tetrachloro-1-1-3-3-tetraethylbenzimidazolylcarbocyanine-iodide-cas-3520-43-2.png)
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 515/529 |
| MW | 652.23 |
| CAS# | 3520-43-2 |
| Solvent | DMSO |
| Storage | F/D/L |
| Category | CellAnalysis CellApoptosis |
| Related | VitalStains NeurologicalStains BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
1.PrepareJC-1workingsolution:
1.1 Preparea2to10mMstocksolutionofJC-1inhigh-quality,anhydrousDMSO.Thestocksolutionshouldbeusedpromptly;anyremainingsolutionshouldbealiquotedandfrozenat<-20oC.
Note:Avoidrepeatedfreeze-thawcycles,andprotectfromlight.
1.2 Preparea1XJC-1workingsolution:Onthedayoftheexperiment,eitherdissolveJC-1solidinDMSOorthawanaliquotoftheJC-1stocksolutiontoroomtemperature.Preparea10to30µM1XJC-1workingsolutioninHanksand20mMHepesbuffer(HHBS)orbufferofyourchoice,pH7with0.02%Pluronic®F-127.Mixthemwellbyvotexing.
Note:JC-1isnotwatersoluble,soitintendstoaggregateinsolution.ItisrecommendedtofiltertheJC-1workingsolutionbeforeloADIngitintothecells.
2.RunJC-1assaywithafluorescencemicroplatereader:
2.1 Treatcellswithtestcompoundsforadesiredperiodoftime(Forexample,Jurkatcellscanbetreatedwithcamptothecinfor4-6hours)toinduceapoptosis.Forblankwells(mediumwithoutthecells),addthecorrespondingamountofcompoundbuffer.
2.2 Add100µL/well/96-wellplateor25µL/well/384-wellplateofJC-1workingsolution(fromStep1.2)intothecellplate.
2.3 IncubatetheJC-1loadingplateina37oC,5%CO2incubatorfor15-60min.
Note:Theappropriateincubationtimedependsontheindividualcelltypeandcellconcentrationused.Optimizetheincubationtimeforeachexperiment.
2.4 RemovetheJC-1workingsolutionfromtheplate,washthecellswithHHBSorbufferofyourchoice.Add100µL/well/96-wellplateor25µL/well/384-wellplateofHHBSbacktothecellplate.
2.5 MonitorthefluorescencechangeatEx/Em=490/525nmand490/590nmforratioanalysis.
3.RunJC-1assaywithafluorescencemicroscopeoraflowcytometer:
3.1 Treatcellswithtestcompoundsforadesiredperiodoftime(Forexample,Jurkatcellscanbetreatedwithcamptothecinfor4-6hours)toinduceapoptosis.
3.2 Centrifugethecellstoget1-5x105cellspertube.
3.3 ResUSPendcellsin500µLofJC-1workingsolution(fromStep1.2).
3.4 Incubateatroomtemperatureor37°Cfor10to30min,protectedfromlight.
3.5 WashthecellswithHHBSorbufferofyourchoice.Resuspendcellsin500µLofHHBStoget1-5x105cellspertube.
3.6 MonitorthefluorescencechangeatEx/Em=490/525nmand490/590nmwithafluorescencemicroscope(usingFITCandTRITCfilters)oraflowcytometer(usingFL1andFL2channels).
| References&Citations |  CitationExplorer |
Hyaluronanpolymericmicellesfortopicaldrugdelivery
Authors:DanielaSmejkalová,TomášMuthny,KristinaNešporová,MartinaHermannová,EvaAchbergerová,GloriaHuerta-Angeles,MarekSvoboda,MartinCepa,VeronikaMachalová,DominikaLuptáková
Journal:CarbohydratePolymers(2017):86--96
Newrutheniumcompoundsbearingsemicarbazone2-formylopyridinemoiety:PlayingwithauxiliaryligandsfortuningthemechanismofBIOLOGicalactivity
Authors:MichalLomzik,OlgaMazuryk,DorotaRutkowska-Zbik,GrazynaStochel,PhilippeCGros,MalgorzataBrindell
Journal:JournalofInorganicBiochemistry(2017)
InhibitionofmTOR"sCatalyticSitebyPKI-587IsaPromisingTherapeuticOptionforGastroenteropancreaticNeuroendocrineTumorDisease
Authors:HelmaFreitag,FriederikeChristen,FlorentineLewens,IrinaGrass,FranziskaBriest,SaraIwaszkiewicz,BrittaSiegmund,PatriciaGrabowski
Journal:Neuroendocrinology(2016)
MesenchymalStemCells-ConditionedMediaAmelioratesDiabeticEndothelialdysfunctionbyImprovingMitochondrialBioenergeticsviatheSirt1/AMPK/PGC-1αPathway
Authors:YujiaYuan,MeimeiShi,LanLi,JingpingLiu,BoChen,YounanChen,XingxingAn,ShuyunLiu,RuixiLuo,DanLong
Journal:ClinicalScience(2016):CS20160235
mTORcomplex-2stimulatesacetyl-CoAanddenovolipogenesisthroughATPcitratelyaseinHER2/PIK3CA-hyperactivebreastcancer
Authors:YaqingChen,JianchangQian,QunHe,HuiZhao,LourdesToral-Barza,CelineShi,XuesaiZhang,JiangWu,KerYu
Journal:Oncotarget(2016):25224--25240
MultipleActiveCompoundsfromViscumalbumL.SynergisticallyConvergetoPromoteApoptosisinEwingSarcoma
Authors:MonikaTwardziok,SusannKleinsimon,JanaRolff,SebastianJäger,AngelikaEggert,GeorgSeifert,CatharinaIDelebinski
Journal:PloSone(2016):e0159749
Ibuprofenenhancestheanticanceractivityofcisplatininlungcancercellsbyinhibitingtheheatshockprotein70
Authors:HEndo,MYano,YOkumura,HKido
Journal:Celldeath&disease(2014):e1027
CR108,anovelvitaminK3derivativeinducesapoptosisandbreasttumorinhibitionbyreactiveoxygenspeciesandmitochondrialdysfunction
Authors:Chun-RuYang,Wei-SiangLiao,Ya-HuiWu,KaliyappanMurugan,ChinpiaoChen,Jui-IChao
Journal:Toxicologyandappliedpharmacology(2013):611--622
Crotonaldehydeinducesapoptosisinalveolarmacrophagesthroughintracellularcalcium,mitochondriaandp53signalingpathways
Authors:Bi-chengYang,Xiu-jiePan,Zhi-huaYang,Feng-junXiao,Xing-yuLiu,Mao-xiangZhu,Jian-pingXie
Journal:TheJournaloftoxicologicalsciences(2013):225--235
MitochondrialP5,amemberofproteindisulphideisomerasefamily,suppressesoxidativestress-inducedcelldeath
Authors:YuShitara,YuichiTonohora,TakahiroGoto,YasuhiroYamada,TakashiMiki,HirokazuMakino,MasanaoMiwa,TohruKomiya
Journal:Journalofbiochemistry(2012):73--85
