Overview | PrinterFriendlyVersion |
Ex/Em(nm) | 480/525 |
MW | 681 |
CAS# | N/A |
Solvent | Water |
Storage | F/D/L |
Category | NeuroBIOLOGy Beta-AmyloidAnalysis |
Related | NeurologicalStains BiochemicalAssays |
Spectrum | AdvancedSpectrumViewer |
1. Treatanimalswithappropriateproceduresasrecommendedbyyouranimalvendors.
2. Perfusetheanimalwith300mLof0.1Mneutralphosphatebuffered10%formalin(4%formaldehyde)viatheascendingaorta,whileclampingoffthedescendingaorta.
3. Postfixthebrainsatleastovernightinthesamefixativesolutionplus20%sucrose.
4. Cutthetissueonafreezingslidingmicrotomeatathicknessof25um.
5. Collectedthesectionsin0.1Mphosphatebuffer,pH7.4.Mountedon2%gelatincoatedslidesandthenairdriedonaslidewarmerat50degreesCforatleasthalfanhour.
6. Immersetheslidesin1%NaOHwith80%alcohol(20mLof5%NaOHto80mLabsolutealcohol)for5minutes,andthenin70%alcoholfor2minutesfollowedby2minutesinddH2O.
7. Transfertheslidesinto0.06%potassiumpermanganatesolutionfor10minutes,shakeitgently.
8. RinsetheslidesinddH2Ofor2minutes.
9. Preparea0.01%Fluoro-Jade®Bstocksolutionbyadding10mgofthedyepowderto100mLofddH2O,mixitwell.
10. Prepare100mLof0.0004%Fluoro-Jade®Bstainingsolutionbyadding4mLofthestocksolutionto96mLof0.1%aceticacid.
11. Staintheslidesinthestainingsolutionfor20minutes,rinsewithddH2OforoneminuteX3times.
12. Draintheslidesverticallyonapapertowel.Drytheslidesonaslidewarmer(~50degreesC)for5-10minuntiltheywerefullydry.
13. Clearthedryslidesbyimmersioninxyleneforatleast1minutebeforecoverslipwithanon-aqueousnon-fluorescentplasticmountingmediasuchasDPX(SigmaChemicalsCo.,St.Louis,MO).
References&Citations | CitationExplorer |
EarlyMinocyclineandLateFK506TreatmentImprovesSurvivalandAlleviatesNeuroinflammation,Neurodegeneration,andBehavioralDeficitsinPrion-InfectedHamsters
Authors:SyedZahidAliShah,DemingZhao,GiulioTaglialatela,SherHayatKhan,TariqHussain,HaodiDong,MengyuLai,XiangmeiZhou,LifengYang
Journal:Neurotherapeutics(2017):1--21
Sodiumbutyrateexertsneuroprotectiveeffectsbyrestoringtheblood-brainbarrierintraumaticbraininjurymice
Authors:HaixiaoLi,JingSun,FangyanWang,GuoqiangDing,WenqianChen,RenchiFang,YeYao,MengqiPang,Zhong-QiuLu,JiamingLiu
Journal:Brainresearch(2016):70--78