Overview | PrinterFriendlyVersion |
Ex/Em(nm) | 485/525 |
MW | 823 |
CAS# | N/A |
Solvent | Water |
Storage | F/D/L |
Category | NeuroBIOLOGy Beta-AmyloidAnalysis |
Related | NeurologicalStains BiochemicalAssays |
Spectrum | AdvancedSpectrumViewer |
1. Tissuesectionsaremountedontopre-treatedslidesandallowedtodryatroomtemperatureovernight,forexample,mountbraintissuesectionsongelatincoatedslidesanddryat50-60oConslidewarmerfor30minutes.
2. Immerseslidesinbasicalcoholsolution(20mLof1%NaOHto80mLof100%ethanol)for5min.
3. Transfertheslidesinto70%ethanolfor2min,andthenintodistilledwaterfor2min.
4. Incubatein0.06%potassiumpermanganatesolutionfor10min.
5. Rinseslidesindistilledwaterfor2min.
6. Incubatetheslidesin0.0001%solutionofFluoro-Jade®Cdissolvedin0.1%aceticacidfor10minutes.
Note1:Tomake0.0001%solutionofFluoro-Jade®C,firstmakinga0.01%stocksolutionofthedyeindistilledwaterandthenadding1mLofthestocksolutionto99mLof0.1%aceticacidvehicle.
Note2:Theworkingsolutionwasusedwithin2hofpreparation.Thestocksolution,whenrefrigerated,canbekeptforlongperiodsbutshouldbediscardedifthesolutionbecomescloudy.
7. Rinsetheslideswithdistilledwaterfor1minperchangefor3times.
8. Drainexcesswaterontoapapertowel,andair-drytheslidesonaslidewarmerat50oCforatleast5min.
9. Cleantheairdriedslidesinxyleneforatleast1min(1-5min).
10. CoverslipwithDPX(FlukaorSigma)nonfluorescentmountingmedia.Donotusepolarcoverslippingmedia,suchasthosethatcontainwater,alcoholorglycerol.
Note1:Forbestpreservationofsections,applyaclearcoatofnailpolisharoundtheedgesofthecoverslip,storeinthecold.
Note2:ToprotectFluor-jadefromphoto-bleaching,mixthesolutionsindimlight.Whentheslidesarestaining,wrapthestainingtraysinaluminumfoil.
References&Citations | CitationExplorer |
EarlyMinocyclineandLateFK506TreatmentImprovesSurvivalandAlleviatesNeuroinflammation,Neurodegeneration,andBehavioralDeficitsinPrion-InfectedHamsters
Authors:SyedZahidAliShah,DemingZhao,GiulioTaglialatela,SherHayatKhan,TariqHussain,HaodiDong,MengyuLai,XiangmeiZhou,LifengYang
Journal:Neurotherapeutics(2017):1--21
Sodiumbutyrateexertsneuroprotectiveeffectsbyrestoringtheblood-brainbarrierintraumaticbraininjurymice
Authors:HaixiaoLi,JingSun,FangyanWang,GuoqiangDing,WenqianChen,RenchiFang,YeYao,MengqiPang,Zhong-QiuLu,JiamingLiu
Journal:Brainresearch(2016):70--78