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主营:主营:研究并生产荧光和发光探针,信号转导研究的试剂
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当前位置: 首页 > 产品中心 > Fluorescent_dyes > AAT Bioquest/Fluoro-Jade®C/23062/5 mg
商品详细AAT Bioquest/Fluoro-Jade®C/23062/5 mg
AAT Bioquest/Fluoro-Jade®C/23062/5 mg
AAT Bioquest/Fluoro-Jade®C/23062/5 mg
商品编号: 23062
品牌: aatbio
市场价: ¥85560.00
美元价: 51336.00
产地: 美国(厂家直采)
公司:
产品分类: 荧光染料
公司分类: Fluorescent_dyes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
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Ex/Em(nm)485/525
MW823
CAS#N/A
SolventWater
StorageF/D/L
CategoryNeuroBIOLOGy
Beta-AmyloidAnalysis
RelatedNeurologicalStains
BiochemicalAssays
Fluoro-Jade®C,likeitspredecessorsFluoro-JadeandFluoro-JadeB,willstainalldegeneratingneurons,regardlessofspecificinsultormechanismofcelldeath.Therefore,thepatternsofneuronaldegenerationseenfollowingexposuretoeithertheglutamateagoNIST,kainicacid,ortheinhibitorofmitochondrialrespiration,3-NPA,werethesameforalloftheFluoro-Jadedyes.However,therewasaqualitativedifferenceinthestainingcharacteristicsofthethreeFluorochromes.Specifically,Fluoro-JadeCexhibitedthegreatestsignaltobackgroundratio,aswellasthehighestresolution.Thistranslatestoastainofmaximalcontrastandaffinityfordegeneratingneurons.Thismakesitidealforlocalizingnotonlydegeneratingnervecellbodies,butalsodistaldendrites,axonsandterminals.ThedyeishighlyresistanttofADIngandiscompatIBLewithvirtuallyallhistologicalprocessingandstainingprotocols.TriplelabelingcanbeaccomplishedbystainingdegeneratingneuronswithFluoro-JadeC,cellnucleiwithDAPIandactivatedastrocyteswithGFAPimmunofluorescence.Fluoro-Jade®isaregisteredtrademarkofHisto-Chem,Inc.
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
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Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.      Tissuesectionsaremountedontopre-treatedslidesandallowedtodryatroomtemperatureovernight,forexample,mountbraintissuesectionsongelatincoatedslidesanddryat50-60oConslidewarmerfor30minutes.

2.      Immerseslidesinbasicalcoholsolution(20mLof1%NaOHto80mLof100%ethanol)for5min.

3.      Transfertheslidesinto70%ethanolfor2min,andthenintodistilledwaterfor2min.

4.      Incubatein0.06%potassiumpermanganatesolutionfor10min.

5.      Rinseslidesindistilledwaterfor2min.

6.      Incubatetheslidesin0.0001%solutionofFluoro-Jade®Cdissolvedin0.1%aceticacidfor10minutes.

Note1:Tomake0.0001%solutionofFluoro-Jade®C,firstmakinga0.01%stocksolutionofthedyeindistilledwaterandthenadding1mLofthestocksolutionto99mLof0.1%aceticacidvehicle.

Note2:Theworkingsolutionwasusedwithin2hofpreparation.Thestocksolution,whenrefrigerated,canbekeptforlongperiodsbutshouldbediscardedifthesolutionbecomescloudy.

7.      Rinsetheslideswithdistilledwaterfor1minperchangefor3times.

8.      Drainexcesswaterontoapapertowel,andair-drytheslidesonaslidewarmerat50oCforatleast5min.

9.      Cleantheairdriedslidesinxyleneforatleast1min(1-5min).

10.   CoverslipwithDPX(FlukaorSigma)nonfluorescentmountingmedia.Donotusepolarcoverslippingmedia,suchasthosethatcontainwater,alcoholorglycerol.

Note1:Forbestpreservationofsections,applyaclearcoatofnailpolisharoundtheedgesofthecoverslip,storeinthecold.

Note2:ToprotectFluor-jadefromphoto-bleaching,mixthesolutionsindimlight.Whentheslidesarestaining,wrapthestainingtraysinaluminumfoil.

References&Citations
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EarlyMinocyclineandLateFK506TreatmentImprovesSurvivalandAlleviatesNeuroinflammation,Neurodegeneration,andBehavioralDeficitsinPrion-InfectedHamsters
Authors:SyedZahidAliShah,DemingZhao,GiulioTaglialatela,SherHayatKhan,TariqHussain,HaodiDong,MengyuLai,XiangmeiZhou,LifengYang
Journal:Neurotherapeutics(2017):1--21

Sodiumbutyrateexertsneuroprotectiveeffectsbyrestoringtheblood-brainbarrierintraumaticbraininjurymice
Authors:HaixiaoLi,JingSun,FangyanWang,GuoqiangDing,WenqianChen,RenchiFang,YeYao,MengqiPang,Zhong-QiuLu,JiamingLiu
Journal:Brainresearch(2016):70--78


品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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