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主营:主营:研究并生产荧光和发光探针,信号转导研究的试剂
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当前位置: 首页 > 产品中心 > Other_kits > AAT Bioquest/细胞仪与贸易;比色抗氧化活性测定试剂盒/15900/200试验
商品详细AAT Bioquest/细胞仪与贸易;比色抗氧化活性测定试剂盒/15900/200试验
AAT Bioquest/细胞仪与贸易;比色抗氧化活性测定试剂盒/15900/200试验
AAT Bioquest/细胞仪与贸易;比色抗氧化活性测定试剂盒/15900/200试验
商品编号: 15900
品牌: aatbio
市场价: ¥85560.00
美元价: 51336.00
产地: 美国(厂家直采)
公司:
产品分类: 其它检测试剂盒
公司分类: Other_kits
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
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Ex/Em(nm)405/None
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategoryNeuroBIOLOGy
ReactiveOxygenSpecies
RelatedCellSignaling
Reactiveoxygenspecies(ROS)areimportantincellsignalingandmaintainingphysiologicalcellmetabolism.ExcessivelevelofROSdamagescellularcomponents,whichlinkstonumerousdiseases,includingcancer,metabolicdisorders,neurodegenerativediseaseetc.Antioxidants,includingenzymes,macromoleculesandsmallmoleculesaregeneratedtocombattheROSinduceddamagesinthelivingorganisms.Quantitativeanalysisofantioxidantactivityinbodyfluids,tissuesandcellsprovidesimportantbiologicalinformation.OurAmplite™ColorimetricantioxidantassaykituseABTSancolorimetricindicatorofantioxidantactivitybasedontheobservationthattheABIlityofantioxidantstopreventtheoxidationofABTS(2,2"-azino-bis(3-ethylbenzothiazoline-6-sulfonicacid)toABTS+byHorserADIshPeroxidase(HRP)andHydrogenperoxide(H2O2).ABTS+,asolublechromogen,canbemonitoredspectrophotometricallyat405nm.
SpectrumAdvancedSpectrumViewer



Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.
  1. Preparestocksolutions:
    1. 2000XPeroxidasestocksolution:Add1mLofAssayBuffer(ComponentB)intothevialofHorseradishPeroxidase(ComponentA).
      Note:TheunusedHRPsolutionshouldbestoredinsingleusealiquotsat-20°C.
    2. 10mMTroloxstocksolution:Add1mlofAssayBuffer(ComponentB)intothevialofTrolox(ComponentD).
      Note:Theunused10mMTroloxstocksolutionshouldbedividedintosingleusealiquotsandstoredat-20°C.

  2. PrepareTroloxstandardsortestsamples:
    1. PrepareaTroloxstandardbydiluting100μLofthe10mMTroloxstocksolution(fromStep1.2)into400μLofAssayBuffer(ComponentB)tohave2mMTroloxstandardsolution.Andthenperform1:2serialdilutionstoget1,0.5,0.25,0.125,0.0625,0.0312and0.0156mMseriallydilutedTroloxstandardsolution.Anon-Troloxbufferisincludedasblankcontrol.

  3. Add10µlTroloxstandards(induplicate)or10µltestsamples(dilutethesamplesinAssayBuffertobringtheantioxidantlevelwithinthesamerangeasTrolox,ifnecessary),or10µlAssayBuffer(BlankControl)ineachwell.
    Table1:LayoutofTroloxstandardsandtestsamplesinaclear96-wellmicroplate
    BLBLTSTS........
    SD1SD1................
    SD2SD2
    SD3SD3
    SD4SD4
    SD5SD5
    SD6SD6
    SD7SD7
    Note:SD=Standards;BL=BlankControl;TS=TestSamples

    Table2:Reagentcompositionforeachwell
    TroloxStandardBlankControlTestSample
    Serialdilutions:10μLAssayBuffer:10μL10μL

  4. AddHRPworkingsolution:
    1. Add2.5uLof2000XHRP(fromStep1.1)into5mLassaybuffer(ComponentB)tomakelHRPworkingsolution.
    2. Add20µlofHRPworkingsolutionineachwell.

  5. Add150µlReadiUseTMABTSSubstrate(ComponentC)Solutiontoeachwell.
    Note:Avoidlightanduseinpolypropylenecontainers.Theunusedsolutioncanbestoredat4°C.

  6. Incubatefor5minutesatroomtemperature.
    Note:The5minutesincubationtimeisaguideline.Theincubationtimecanbechangedtoobtainsuitableabsorbance.

  7. Readtheabsorbanceat405nmusinganAbsorbancemicroplatereader.
References&Citations
CitationExplorer

Fluorescentreal-timequantitativemeasurementsofintracellularperoxynitritegenerationandinhibition
Authors:ZhenLuo,QinZhao,JixiangLiu,JinfangLiao,RuoguPeng,YuntingXi,ZhenjunDiwu
Journal:Analyticalbiochemistry(2017):44--48

GenesilencingofendothelialvonWillebrandFactorattenuatesangiotensinII-inducedendothelin-1expressioninporcineaorticendothelialcells
Authors:AnarDushpanova,SilviaAgostini,EnricaCiofini,ManuelaCabiati,ValentinaCasieri,MarcoMatteucci,SilviaDelRy,AldoClerico,SergioBerti,VincenzoLionetti
Journal:ScientificReports(2016)


品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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