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AAT Bioquest/BrdU [5-Bromo-2'-deoxyuridine] *CAS 59-14-3*/17030/25 mg
![AAT Bioquest/BrdU [5-Bromo-2'-deoxyuridine] *CAS 59-14-3*/17030/25 mg](https://www.ebiomall.cn/images/AAT201712/chemical-structure-for-brdu-5-bromo-2-deoxyuridine-cas-59-14-3.png)
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | None/None |
| MW | 307.09 |
| CAS# | 59-14-3 |
| Solvent | DMSO |
| Storage | F/D/L |
| Category | CellBIOLOGy LabelingCells |
| Related | RNADetection BiochemicalAssays |
1).Make1-10mMDMSOstocksolution.TheDMSOstocksolutionisgoodfor6monthsifstoredat-20°C.
2).PelletcellsbycentrifugationandresUSPendthecellsincompletedgrowthmedia.Adherentcellsinculturemaybestainedinsituoncoverslipsorinthecellculturewells.
3).AddBrdustainusingtheconcentrationsbetween5and20µMandincubateitfor60minutestoovernightasaguide.Theoptimumincubationtimewilldependuponcelltypeandthegoaloftheexperiment.
4).Fixcellsbywith70-80%alcoholoracid-ethanolfor20-30min.
5).WashwithPBS(3times,2min.each).
6).Proceedwithimmunocytochemicalstainingtechniques.
| References&Citations |  CitationExplorer |
Heat-shockpre-treatmentreducesliverinjuryandaidsliverrecoveryafterpartialhepatectomyinmice
Authors:YousukeOka,YoshitoAkagi,TetsushiKinugasa,NobuyaIshibashi,NobutakaIwakuma,IchitarouShiratsuchi,KazuoShirouzu
Journal:Anticancerresearch(2013):2887--2894
