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AAT Bioquest/Acridine orange *10 mg/mL solution in water*/17503/10 mL
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 500/526 |
| MW | 301.82 |
| CAS# | 65-61-2 |
| Solvent | Water |
| Storage | F/D/L |
| Category | NucleicAcidDetection DNADetection |
| Related | LabelingCells BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
1).Make1-10mMDMSOstocksolution.TheDMSOstocksolutionisgoodfor6monthsifstoredat-20°C.
2).Usethefixationprotocolappropriateforyoursample.
3).PelletcellsbycentrifugationandresUSPendthecellsinbufferedsaltsolutionsormedia,withoptimaldyestainingatpH7.4.Adherentcellsinculturemaybestainedinsituoncoverslipsorinthecellculturewells.
4).AddAOstainusingtheconcentrationsbetween1and10µMandincubateitfor15to60minutesasaguide.
Note:Ininitialexperiments,itmaybebesttotryseveraldyeconcentrationsovertheentiresuggestedrangetodeterminetheconcentrationthatyieldsoptimalstaining.
| References&Citations |  PrinterFriendlyVersion |
1. ShiY,ZhengWW,WuXX,ZhouDH.(2006)[Theinteractionofacridineorangewithanionicsurfactantanditsapplicationtothedeterminationofprotein].GuangPuXueYuGuangPuFenXi,26,1653.
2. HirumaH,KatakuraT,TakenamiT,IgawaS,KanohM,FujimuraT,KawakamiT.(2006)Vesicledisruption,plasmamembraneblebformation,andacutecelldeathcausedbyIlluminationwithbluelightinacridineorange-loadedmalignantmelanomacells.JPhotochemPhotobiolB.
3. KrolenkoSA,AdamyanSY,BelyaevaTN,MozhenokTP.(2006)Acridineorangeaccumulationinacidorganellesofnormalandvacuolatedfrogskeletalmusclefibres.CellBiolInt,30,933.
4. SantiwatanakulS,KriegNR.(2005)AcridineorangestainingandviABIlityofthecoccoidformofCampylobacterupsaliensis.JMedAssocThai,88Suppl1,S5.
5. CelikA,OgenlerO,ComelekogluU.(2006)Re:Theevaluationofmicronucleusfrequencybyacridineorangefluorescentstaininginperipheralbloodofratstreatedwithleadacetate.(Mutagenesis,20,411-415,2005).Mutagenesis,21,267.
6. LiuBS,GaoJ,LiuZC,YuLN,YangDC,YangGL.(2006)[Fluorescenceresonanceenergytransferquenchingmethodfordeterminationofarsenicwithacridineorange-rhodamineB].GuangPuXueYuGuangPuFenXi,26,306.
7. ProudlockRJ.(2006)Re:Theevaluationofmicronucleusfrequencybyacridineorangefluorescentstaininginperipheralbloodofratstreatedwithleadacetate.(Mutagenesis,20,411-415,2005).Mutagenesis,21,265;authorreply267.
8. ValentiniA,PucciD,CrispiniA,FedericiG,BernardiniS.(2006)AcridineOrangebasedplatinum(II)complexesinducingcytotoxicityandcellcycleperturbationinspiteofGSTP1up-regulation.ChemBiolInteract,161,241.
9. WangF,YangJ,WuX,WangX,GuoC,JiaZ.(2006)Improvementoftheacridineorange-protein-surfactantsystemforproteinestimationbasedonaromaticringstackingeffectofsodiumdodecylbenzenesulphonate.Luminescence,21,186.
10. FuseH,AkashiT,MizunoI,NozakiT,WatanabeA.(2006)Postoperativechangesofspermchromatinheterogeneity,usingacridineorangestaining,invaricocelepatients.ArchAndrol,52,223.
