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						AAT Bioquest/Live or Dead™ Fixable Dead Cell Staining Kit *Blue Fluorescence with 405 nm Excitation*/22500/200 Tests
					
								| Overview | PrinterFriendlyVersion  | 
| Ex/Em(nm) | 410/450 | 
| MW | N/A | 
| CAS# | N/A | 
| Solvent | DMSO | 
| Storage | F/D/L | 
| Category | CellBIOLOGy LabelingCells  | 
| Related | FluorescenceImaging BiochemicalAssays  | 
| Spectrum | AdvancedSpectrumViewer | 

1) Add200µLDMSO(ComponentB)intothevialofStainIt™ReactiveDye(ComponentA)tohave500XDMSOstocksolution.
Note:TheunusedStainIt™dyestocksolutionshouldbedividedintosingleusealiquotsandstoredat‑20 oC.Avoidrepeatedfreeze/thawcycles.
2) Preparecellsforflowcytometrystainingusing1XHanksand20mMHepesbuffer(HHBS)orsodiumazide-freeandserum/protein-freebufferofyourchoice.
3) WashcellsoncewithHHBSortheazide-andserum/protein-freebufferofyourchoice.
4) ResUSPendcellsat5-10×106/mLinHHBSorintheazide-andserum/protein-freebufferofyourchoice.
5) Add1µLofStainIt™ReactiveDyestocksolution(fromStep1)to0.5mLofcells/assayandmixitwell.
6) Incubatefor20to60minatroomtemperatureor37°C,5%CO2incubator,protectedfromlight.
Note:Theoptimalstainconcentrationsandincubationtimeshouldbeexperimentallydeterminedfordifferentcelllines.
7) WashcellstwiceandresuspendcellswithHHBSorthebufferofyourchoice.
8) Fixcellsasdesired(optional).
9) Analyzecellswithflowcytometer.
| References&Citations | CitationExplorer  | 
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