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AAT Bioquest/Live or Dead™ Fixable Dead Cell Staining Kit *Deep Red Fluorescence*/22604/200 Tests
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 649/660 |
| MW | N/A |
| CAS# | N/A |
| Solvent | N/A |
| Storage | F/D/L |
| Category | CellBIOLOGy LabelingCells |
| Related | FluorescenceImaging BiochemicalAssays |
1) Add200µLDMSO(ComponentB)intothevialofStainIt™ReactiveDye(ComponentA)tohave500XDMSOstocksolution.
Note:TheunusedStainIt™dyestocksolutionshouldbedividedintosingleusealiquotsandstoredat‑20 oC.Avoidrepeatedfreeze/thawcycles.
2) Preparecellsforflowcytometrystainingusing1XHanksand20mMHepesbuffer(HHBS)orsodiumazide-freeandserum/protein-freebufferofyourchoice.
3) WashcellsoncewithHHBSortheazide-andserum/protein-freebufferofyourchoice.
4) ResUSPendcellsat5-10×106/mLinHHBSorintheazide-andserum/protein-freebufferofyourchoice.
5) Add1µLofStainIt™ReactiveDyestocksolution(fromStep1)to0.5mLofcells/assayandmixitwell.
6) Incubatefor20to60minatroomtemperatureor37°C,5%CO2incubator,protectedfromlight.
Note:Theoptimalstainconcentrationsandincubationtimeshouldbeexperimentallydeterminedfordifferentcelllines.
7) WashcellstwiceandresuspendcellswithHHBSorthebufferofyourchoice.
8) Fixcellsasdesired(optional).
9) Analyzecellswithflowcytometer.
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