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Fluorescent_dyes >
AAT Bioquest/Cell Navigator™ Live Cell RNA Imaging Kit *Green Fluorescence*/22630/100 Tests
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 503/511 |
| MW | N/A |
| CAS# | N/A |
| Solvent | N/A |
| Storage | F/D/L |
| Category | CellBIOLOGy LabelingCells |
| Related | FluorescenceImaging BiochemicalAssays |
1. Culturecellstoadensityoptimumforimagingaccordingtoyourspecificinductionprotocol(about1-2×104cells/well/96-wellplate).
2. Forlivingcells:IncubatecellswithStrandBrite™RNAGreen(ComponentA)diluted400Xinmediumorlivecellstainingbuffer(ComponentB)atroomtemperaturefor30-60minutes(100µL/well).
Note1:25μLofStrandBrite™RNAGreen(ComponentA)isenoughforone96-wellplate.AliquotandstoreunusedStrandBrite™RNAGreenat<-20ºC.Protectfromlightandavoidrepeatedfreeze-thawcycles.
Note2:Theappropriateincubationtimedependsontheindividualcelltypeandcellconcentrationused.Optimizetheincubationtimeforeachexperiment.Seefigure1fordetails.
3. Forfixedcells:Fixcellswithpuremethanolfor1minuteatroomtemperature,thenwashwithPBS.Immersecellsin1%Triton-100for2minutes,thenwashwithPBStwice.IncubatecellswithStrandBrite™RNAGreen(ComponentA)attheconcentrationof1XinPBSatroomtemperaturefor15-30minutes.
Note:Itisrecommendedtoincreaseeitherthelabelingconcentrationortheincubationtimetoallowthedyetoaccumulateifthecellsdonotappeartobesufficientlystained.Seefigure1fordetails.
4. (Optional)WashthecellswithPBSfor1-2times,add100μLPBStoeachwell.
5. MonitorfluorescentintensitywithafluorescencemicroscopeatEx/Em=490/520nm(FITCchannel).
