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AAT Bioquest/Cell Navigator™ F-Actin Labeling Kit *Blue Fluorescence*/22660/500 Tests
| Overview |
 Printer Friendly Version
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| Ex/Em (nm) | 353/442 |
| MW | N/A |
| CAS # | N/A |
| Solvent | N/A |
| Storage | F/D/L |
| Category |
Cell Biology Labeling Cells |
| Related |
Fluorescence Imaging Biochemical Assays |
| Spectrum | Advanced Spectrum Viewer |
1. Prepare 1X iFluor™ 350-Phalloidin working solution:
Add 10 µL of iFluor™ 350-Phalloidin (Component A) to 10 mL of Labeling Buffer (Component B).
Note 1: The unused iFluor™ 350-Phalloidin stock solution (Component A) should be aliquoted and stored at -20 ºC. Protect from light.
Note 2: Different cell types might be stained differently. The concentration of iFluor™ 350-Phalloidin working solution should be prepared accordingly.
2. Stain the cells:
2.1 Perform formaldehyde fixation. Incubate the cells with 3.0–4.0 % formaldehyde in PBS at room temperature for 10–30 minutes.
Note: Avoid any methanol containing fixatives since methanol can disrupt actin during the fixation process. The preferred fixative is methanol-free formaldehyde.
2.2 Rinse the fixed cells 2–3 times in PBS.
2.3 Optional: Add 0.1% Triton X-100 in PBS into fixed cells (from Step 2.2) for 3 to 5 minutes to increase permeability. Rinse the cells 2–3 times in PBS.
2.4 Add 100 μL/well (96-well plate) of 1X iFluor™ 350-Phalloidin working solution (from Step 1) into the fixed cells (from Step 2.2 or 2.3), and stain the cells at room temperature for 15 to 60 minutes.
2.5 Rinse cells gently with PBS 2 to 3 times to remove excess dye before plate sealing and imaging by using DAPI channel.
| References & Citations |
 Citation Explorer
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Porous Li-containing biphasic calcium phosphate scaffolds fabricated by three-dimensional plotting for bone repair
Authors: Xiaoheng Guo, Huichang Gao, Xiao Liu, Jingjing Diao, Xuetao Shi, Naru Zhao, Yingjun Wang
Journal: RSC Advances (2017): 34508--34516
Preparation, characterization and in vitro cell performance of anti-washout calcium phosphate cement modified by sodium polyacrylate
Authors: Xingmei Li, Fupo He, Jiandong Ye
Journal: RSC Advances (2017): 32842--32849
miR-29b-Loaded Gold Nanoparticles Targeting to the Endoplasmic Reticulum for Synergistic Promotion of Osteogenic Differentiation
Authors: Ting Pan, Wenjing Song, Huichang Gao, Tianjie Li, Xiaodong Cao, Shizhen Zhong, Yingjun Wang
Journal: ACS Applied Materials & Interfaces (2016): 19217--19227
Osteogenic and tenogenic induction of hBMSCs by integrated nanofibrous scaffold with chemical and structural mimic to bone-ligament connection
Authors: Zifeng Lin, Xiujuan Zhao, Si Chen, Chang Du
Journal: Journal of Materials Chemistry B (2016)
The stimulation of the differentiation of pheochromocytoma (PC12-L) cells into neuron-like cells by electrically conductive nanofibre mesh
Authors: Huishang Yang, Guanglin Zhu, Yicheng Huang, Xuetao Shi, Yingjun Wang
Journal: Applied Materials Today (2016): 215--222
Distinct mechanical behavior of HEK293 cells in adherent and suspended states
Authors: Seyed Mohammad Ali Haghparast, Takanori Kihara, Jun Miyake
Journal: PeerJ (2015): e1131
The preparation and characterization of polycaprolactone/graphene oxide biocomposite nanofiber scaffolds and their application for directing cell behaviors
Authors: Juqing Song, Huichang Gao, Guanglin Zhu, Xiaodong Cao, Xuetao Shi, Yingjun Wang
Journal: Carbon (2015): 1039--1050
Actin-based biomechanical features of suspended normal and cancer cells
Authors: Seyed Mohammad Ali Haghparast, Takanori Kihara, Yuji Shimizu, Shunsuke Yuba, Jun Miyake
Journal: Journal of bioscience and bioengineering (2013): 380--385
