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AAT Bioquest/Cell Navigator&trade；F-肌动蛋白标记试剂盒*红色荧光*/22664/500测试
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 583/603 |
| MW | N/A |
| CAS# | N/A |
| Solvent | N/A |
| Storage | F/D/L |
| Category | CellBIOLOGy LabelingCells |
| Related | FluorescenceImaging BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
1.Prepare1XiFluor™594-Phalloidinworkingsolution:
Add10µLofiFluor™594-Phalloidin(ComponentA)to10mLofLabelingBuffer(ComponentB).
Note1:TheunusediFluor™594-Phalloidinstocksolution(ComponentA)shouldbealiquotedandstoredat-20ºC.Protectfromlight.
Note2:Differentcelltypesmightbestaineddifferently.TheconcentrationofiFluor™594-Phalloidinworkingsolutionshouldbepreparedaccordingly.
2.Stainthecells:
2.1 Performformaldehydefixation.Incubatethecellswith3.0–4.0%formaldehydeinPBSatroomtemperaturefor10–30minutes.
Note:Avoidanymethanolcontainingfixativessincemethanolcandisruptactinduringthefixationprocess.Thepreferredfixativeismethanol-freeformaldehyde.
2.2 Rinsethefixedcells2–3timesinPBS.
2.3 Optional:Add0.1%TritonX-100inPBSintofixedcells(fromStep2.2)for3to5minutestoincreasepermeability.Rinsethecells2–3timesinPBS.
2.4 Add100μL/well(96-wellplate)ofiFluor™594-Phalloidinworkingsolution(fromStep1)intothefixedcells(fromStep2.2or2.3),andstainthecellsatroomtemperaturefor15to60minutes.
2.5 RinsecellsgentlywithPBS2to3timestoremoveexcessdyebeforeplatesealingandimagingbyusingTexasRedchannel(Ex/Em=594/610nm).
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