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主营:主营:研究并生产荧光和发光探针,信号转导研究的试剂
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当前位置: 首页 > 产品中心 > Other_kits > AAT Bioquest/Cell Navigator™ Mitochondrion Staining Kit *Orange Fluorescence*/22667/500 Assays
商品详细AAT Bioquest/Cell Navigator™ Mitochondrion Staining Kit *Orange Fluorescence*/22667/500 Assays
AAT Bioquest/Cell Navigator™ Mitochondrion Staining Kit *Orange Fluorescence*/22667/500 Assays
AAT Bioquest/Cell Navigator™ Mitochondrion Staining Kit *Orange Fluorescence*/22667/500 Assays
商品编号: 22667
品牌: aatbio
市场价: ¥56560.00
美元价: 33936.00
产地: 美国(厂家直采)
公司:
产品分类: 其它检测试剂盒
公司分类: Other_kits
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Overview
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Ex/Em(nm)545/575
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategoryCellBIOLOGy
LabelingCells
RelatedFluorescenceImaging
BiochemicalAssays
OurCellNavigator™fluorescenceimagingkitsareasetoffluorescenceimagingtoolsforlabelingsub-cellularorganellessuchasmembranes,lysosomes,mitochondriaandnucleietc.Theselectivelabelingoflivecellcompartmentsprovidesapowerfulmethodforstudyingcellulareventsinaspatialandtemporalcontext.Thisparticularkitisdesignedtolabelmitochondriaoflivecellsinorangefluorescence.ThecellimagescanbeviewedwiththeTRITCfilterset.ThekitusesaproprietarydyethatselectivelyaccumulatesinmitochondriaprobablyvialthemitochondrialmembranepotentialgrADIent.Themitochondrialindicatorisahydrophobiccompoundthateasilypermeatesintactlivecells,andtrappedinmitochondriaafteritgetsintocells.Thisfluorescentmitochondrialindicatorisretainedinmitochondriaforlongtimesincetheindicatorcarriesacell-retaininggroup.Thiskeyfeaturesignificantlyincreasesitsstainingefficiency.Thelabelingprotocolisrobust,requiringminimalhands-ontime.Itcanbereadilyadaptedforawidevarietyoffluorescenceplatformssuchasmicroplateassays,immunocytochemistryandflowcytometry.Itisusefulforavarietyofstudies,includingcelladhesion,chemotaxis,multidrugresistance,cellviABIlity,apoptosisandcytotoxicity.Thekitprovidesalltheessentialcomponentswithanoptimizedcell-labelingprotocol.Itissuitableforproliferatingandnon-proliferatingcells,andcanbeusedforbothsUSPensionandadherentcells.
SpectrumAdvancedSpectrumViewer

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Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.Preparemitochondrialstainingsolution:

1.1   Warmallthecomponentstoroomtemperature.

1.2   Preparedyeworkingsolutionbydiluting20µLofMitolite™Orange(ComponentA)into10mLofLiveCellStainingBuffer(ComponentB).

Note1:20µLof500XMitolite™Orange(ComponentA)isenoughforone96-wellplate.Aliquotandstoreunused500XMitolite™Orangeat<-20oC.Protectfromlightandavoidrepeatedfreeze-thawcycles.

Note2:Theoptimalconcentrationofthefluorescentmitochondrialindicatorvariesdependingonthespecificapplication.Thestainingconditionsmaybemodifiedaccordingtotheparticularcelltypeandthepermeabilityofthecellsortissuestotheprobe.

 

2.Prepareandstaincells:

2.1   Foradherentcells:Growcellseitherina96-wellblackwall/clearbottomplateoroncover-slipsinsideapetridishfilledwiththeappropriateculturemedium.Whencellsreachthedesiredconfluence,addequalvolume(e.g.100µLfora96-wellplateand25µLfora384-wellplate)ofthedye-workingsolution(fromStep1.2).Incubatethecellsina37°C,5%CO2incubatorfor30minutesto2hours.Replacethedye-loadingsolutionwithHanksand20mMHepesbuffer(HHbuffer)orbufferofyourchoice(e.g.thebufferwithgrowthmediumat1:1concentration).ObservethecellsbyusingafluorescencemicroscopefittedwithaTRTICfilterset.

Note:Itisrecommendedtoincreaseeitherthelabelingconcentrationortheincubationtimetoallowthedyetoaccumulateifthecellsdonotappeartobesufficientlystained.

2.2    Forsuspensioncells:Centrifugethecellsat1000rpmfor5minutestoobtainacellpelletandaspiratethesupernatant.Resuspendthecellpelletsgentlyinpre-warmed(37°C)growthmedium,andaddequalvolumeofthedye-workingsolution(fromStep1.2).Incubatethecellsina37°C,5%CO2incubatorfor30minutesto2hours.Replacethedye-loadingsolutionwithHanksand20mMHepesbuffer(HHbuffer)orbufferofyourchoice(e.g.thebufferwithgrowthmediumat1:1concentration).ObservethecellsusingafluorescencemicroscopefittedwithaTRITCfilterset.

Note1:Itisrecommendedtoincreaseeitherthelabelingconcentrationortheincubationtimetoallowthedyetoaccumulateifthecellsdonotappeartobesufficientlystained.

Note2:Suspensioncellsmaybeattachedtocover-slipsthathavebeentreatedwithBDCell-Tak®(BDBiosciences)andstainedasadherentcells(seeStep2.1).

References&Citations
CitationExplorer

Co-deliveryofVP-16andBcl-2-targetedantisenseonPEG-graftedoMWCNTsforsynergisticinvitroanti-cancereffectsinnon-smallandsmallcelllungcancer
Authors:ZbynekHeger,HanaPolanska,SonaKrizkova,JanBalvan,MartinaRaudenska,SimonaDostalova,AmitavaMoulick,MichalMasarik,VojtechAdam
Journal:ColloidsandSurfacesB:Biointerfaces(2017):131--140

Inhibitionofhemeoxygenase-1enhancesthechemosensitivityoflaryngealsquamouscellcancerHep-2cellstocisplatin
Authors:XinLv,Dong-meiSong,Ying-haoNiu,Bao-shanWang
Journal:Apoptosis(2016):489--501

Effectivetwo-photonexcitedphotodynamictherapyofxenografttumorssensitizedbywater-solublebis(arylidene)cycloalkanonephotosensitizers
Authors:QianliZou,HongyouZhao,YuxiaZhao,YanyanFang,DefuChen,JieRen,XiaopuWang,YingWang,YingGu,FeipengWu
Journal:Journalofmedicinalchemistry(2015):7949--7958

MelatoninpromotesAdipogenesisandmitochondrialbiogenesisin3T3-L1preadipocytes
Authors:HisashiKato,GokiTanaka,ShinyaMasuda,JunetsuOgasawara,TakuyaSakurai,TakakoKizaki,HidekiOhno,TetsuyaIzawa
Journal:JournalofPinealResearch(2015):267--275


品牌介绍
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。 1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物; 2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞; 3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类; 4)致力于开发用于信号转导研究的试剂; 5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
品牌分类
淋巴细胞信号传导 GPCR抗体 羰基活性生物素 细胞代谢 胺反应生物素 链霉亲和素结合物 生物素化抗IgGs 乙酰基特异性抗体 细胞骨架抗体
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