Overview | PrinterFriendlyVersion |
Ex/Em(nm) | 660/693 |
MW | N/A |
CAS# | N/A |
Solvent | N/A |
Storage | F/D/L |
Category | CellBIOLOGy LabelingCells |
Related | FluorescenceImaging |
Spectrum | AdvancedSpectrumViewer |
- Preparemitochondrialstainingsolution:
- Warmallthecomponentstoroomtemperature.
- Preparedyeworkingsolutionbydiluting20μLofMitolite™NIR(ComponentA)into10mLofLiveCellStainingBuffer(ComponentB).
Note1:20µLof500XMitolite™NIR(ComponentA)isenoughforone96-wellplate.Aliquotandstoreunused500XMitolite™NIRat≤-20ºC.Protectfromlightandavoidrepeatedfreeze-thawcycles.
Note2:Theoptimalconcentrationofthefluorescentmitochondrialindicatorvariesdependingonthespecificapplication.Thestainingconditionsmaybemodifiedaccordingtotheparticularcelltypeandthepermeabilityofthecellsortissuestotheprobe.
- Prepareandstaincells:
- Foradherentcells:Growcellseitherina96-wellblackwall/clearbottomplateoroncover-slipsinsideapetridishfilledwiththeappropriateculturemedium.Whencellsreachthedesiredconfluence,addequalvolume(e.g.100μLfora96-wellplateand25μLfora384-wellplate)ofthedye-workingsolution(fromStep1.2).Incubatethecellsina37ºC,5%CO2incubatorfor30minutesto2hours.ReplacethedyeloadingsolutionwithHanksand20mMHepesbuffer(HHbuffer)orbufferofyourchoice(e.g.thebufferwithgrowthmediumat1:1concentration).ObservethecellsusingafluorescencemicroscopefittedwithaCy5filterset.
Note:Itisrecommendedtoincreaseeitherthelabelingconcentrationortheincubationtimetoallowthedyetoaccumulateifthecellsdonotappeartobesufficientlystained. - ForsUSPensioncells:Centrifugethecellsat1000rpmfor5minutestoobtainacellpelletandaspiratethesupernatant.Resuspendthecellpelletsgentlyinpre-warmed(37ºC)growthmedium,andaddequalvolumeofthedye-workingsolution(fromStep1.2).Incubatethecellsina37ºC,5%CO2incubatorfor30minutesto2hours.Replacethedye-loadingsolutionwithHanksand20mMHepesbuffer(HHbuffer)orbufferofyourchoice(e.g.thebufferwithgrowthmediumat1:1concentration).ObservethecellsusingafluorescencemicroscopefittedwithaCy5filterset.
Note1:Itisrecommendedtoincreaseeitherthelabelingconcentrationortheincubationtimetoallowthedyetoaccumulateifthecellsdonotappeartobesufficientlystained.
Note2:Suspensioncellsmaybeattachedtocover-slipsthathavebeentreatedwithBDCell-Tak®(BDBiosciences)andstainedasadherentcells(seeStep2.1).
- Foradherentcells:Growcellseitherina96-wellblackwall/clearbottomplateoroncover-slipsinsideapetridishfilledwiththeappropriateculturemedium.Whencellsreachthedesiredconfluence,addequalvolume(e.g.100μLfora96-wellplateand25μLfora384-wellplate)ofthedye-workingsolution(fromStep1.2).Incubatethecellsina37ºC,5%CO2incubatorfor30minutesto2hours.ReplacethedyeloadingsolutionwithHanksand20mMHepesbuffer(HHbuffer)orbufferofyourchoice(e.g.thebufferwithgrowthmediumat1:1concentration).ObservethecellsusingafluorescencemicroscopefittedwithaCy5filterset.
References&Citations | CitationExplorer |
Co-deliveryofVP-16andBcl-2-targetedantisenseonPEG-graftedoMWCNTsforsynergisticinvitroanti-cancereffectsinnon-smallandsmallcelllungcancer
Authors:ZbynekHeger,HanaPolanska,SonaKrizkova,JanBalvan,MartinaRaudenska,SimonaDostalova,AmitavaMoulick,MichalMasarik,VojtechAdam
Journal:ColloidsandSurfacesB:Biointerfaces(2017):131--140
Inhibitionofhemeoxygenase-1enhancesthechemosensitivityoflaryngealsquamouscellcancerHep-2cellstocisplatin
Authors:XinLv,Dong-meiSong,Ying-haoNiu,Bao-shanWang
Journal:Apoptosis(2016):489--501
Effectivetwo-photonexcitedphotodynamictherapyofxenografttumorssensitizedbywater-solublebis(arylidene)cycloalkanonephotosensitizers
Authors:QianliZou,HongyouZhao,YuxiaZhao,YanyanFang,DefuChen,JieRen,XiaopuWang,YingWang,YingGu,FeipengWu
Journal:Journalofmedicinalchemistry(2015):7949--7958
MelatoninpromotesAdipogenesisandmitochondrialbiogenesisin3T3-L1preadipocytes
Authors:HisashiKato,GokiTanaka,ShinyaMasuda,JunetsuOgasawara,TakuyaSakurai,TakakoKizaki,HidekiOhno,TetsuyaIzawa
Journal:JournalofPinealResearch(2015):267--275