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AAT Bioquest/Amplite™ Colorimetric Caspase 3/7 Assay Kit *Yellow Color*/13507/200 Tests
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 498/None |
| MW | N/A |
| CAS# | N/A |
| Solvent | N/A |
| Storage | F/D/L |
| Category | EnzymeDetection PeptidasesandProteases |
| Related | MicroplateReaders |
| Spectrum | AdvancedSpectrumViewer |
- PrepareCells:
- Fornon-adherentcells:CentrifugethecellsfromtheculturemediumandthensUSPendthecellpelletinculturemediumat1,000,000to5,000,000cells/mLfora96-wellpoly-Dlysineplate(100µL/well).Centrifugetheplateat800rpmfor2minuteswithbrakeoffpriortotheexperiments.
Note:Eachcelllineshouldbeevaluatedonanindividualbasistodeterminetheoptimalcelldensityforapoptosisinduction.
- Fornon-adherentcells:CentrifugethecellsfromtheculturemediumandthensUSPendthecellpelletinculturemediumat1,000,000to5,000,000cells/mLfora96-wellpoly-Dlysineplate(100µL/well).Centrifugetheplateat800rpmfor2minuteswithbrakeoffpriortotheexperiments.
- PrepareCaspase3/7assaysolution:
- Add50μLof200Xcaspase3/7Substratestocksolution(ComponentA)into10mLAssayBuffer(ComponentB),andmixwell.
Note:50μLofthe200Xcaspase3/7Substratestocksolutionisenoughfor100assaysusingareactionvolumeof100μLperassay.Theunused200Xcaspase3/7Substratestocksolutionshouldbealiquoted,storeddesiccatedat-20°C,andprotectedfromlight.
- Add50μLof200Xcaspase3/7Substratestocksolution(ComponentA)into10mLAssayBuffer(ComponentB),andmixwell.
- RunCaspase3/7assay:
- Treatcellsbyadding10μLof10Xtestcompounds(96-wellplate)intoPBSordesiredbuffer.Forblankwells(mediumwithoutthecells),addthecorrespondingamountofcompoundbuffer.
- Incubatethecellplatesinanincubatorforadesiredperiodoftimetoinduceapoptosis.
Note:WetreatedJurkatcellswithstaurosporine(SS)for4hoursat37ºCtoinducecellapoptosis.SeeFigure1fordetails. - Add100μL/wellofcaspase3/7assaysolution(fromstep2).
- Incubatetheplateatroomtemperatureforatleast1hour,keptfromlight.
- Centrifugecellplatesat800rpmfor2minuteswithbrakeoff.
- MonitortheabsorbanceincreasewithanabsorbanceplatereaderatOD=490nm.
| References&Citations |  CitationExplorer |
HighSTMN1levelisassociatedwithchemo-resistanceandpoorprognosisingastriccancerpatients
Authors:TuyaBai,TakehikoYokobori,BolagAltan,MunenoriIde,EritoMochiki,MitsuhiroYanai,AkiharuKimura,NorimichiKogure,ToruYanoma,MasakiSuzuki
Journal:BritishJournalofCancer(2017)
Thetrivalentcerium-inducedcelldeathandalterationofionfluxinsweetpotato[Ipomoeabatatas(L.)Lam]
Authors:JiaojiaoJiang,JianzhongHu,ZeyiXie,QingheCao,DaifuMa,YonghuaHan,ZongyunLi
Journal:JournalofRareEarths(2017)
UncarboxylatedOsteocalcinInducesAntitumorImmunityagainstMouseMelanomaCellGrowth
Authors:YoshikazuHayashi,TomoyoKawakubo-Yasukochi,AkikoMizokami,MaiHazekawa,TomikoYakura,MunekazuNaito,HiroshiTakeuchi,SeijiNakamura,MasatoHirata
Journal:JournalofCancer(2017):2478--2486
DocetaxelinducesBcl-2-andpro-apoptoticcaspase-independentdeathofhumanprostatecancerDU145cells
Authors:TakeharuOgura,YoshiyukiTanaka,HirokiTamaki,MamoruHarada
Journal:Internationaljournalofoncology(2016):2330--2338
NT1014,anovelbiguanide,inhibitsovariancancergrowthinvitroandinvivo
Authors:LuZhang,JianjunHan,AmandaLJackson,LeslieNClark,JoshuaKilgore,HuiGuo,NickLivingston,KennethBatchelor,YajieYin,TimothyPGilliam
Journal:JournalofHematology&Oncology(2016):91
Identificationofanallostericsmall-moleculeinhibitorselectivefortheinducibleformofheatshockprotein70
Authors:MatthewKHowe,KhaldonBodoor,DavidACarlson,PhilipFHughes,YazanAlwarawrah,DavidRLoiselle,AlexMJaeger,DavidBDarr,JamieLJordan,LucasMHunter
Journal:Chemistry&BIOLOGy(2014):1648--1659
