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AAT Bioquest/Pluronic® F-127 *Cell culture tested */20050/10 g
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | None/None |
| MW | ~13kD |
| CAS# | N/A |
| Solvent | Water |
| Storage | RT |
| Category | GPCR CalciumGPCRAssays |
| Related | CellSignaling BiochemicalAssays |
- Dissolve1gofPluronic®F-127in10mLdistilledwatertomakea10%(w/v)stocksolution,or2gofPluronic®F-127in10mLofanhydrousdimethylsulfoxide(DMSO)tomakea20%(w/v)stocksolution.Thesemayrequireheatingfrom40to50°Cforabout30minutes.Storesolutionatroomtemperature.
Note:DonotrefrigerateorfreezethePluronic®F-127solutionsinceitmayprecipitate.Ifprecipitationisobserved,theprecipitatescanbedissolvedbyheatingto37°Candvortexingbeforeuse.
- Dilutethe10%or20%Pluronic®F-127stocksolution(fromStep1)intothecell-loADIngbuffersuchasHanksand20mMHepesbuffer(HHBS)at1:1000to1:500dilutiontoachievea0.02to0.04%workingsolution.
- TheDMSOstocksolutionofAMesteristhendilutedintothe0.02to0.04%workingsolution(fromStep 2)toachieveafinalAMesterconcentrationbetween1µMand10µM.
Note:ThefinalconcentrationofPluronic®F-127isnormallykeptatorbelow0.08%.
- Thecellsareincubatedatadesiredtemperatureforbetween10minutesand1hour.
Note:IngeneralitisdesirabletousetheminimumamountofAMesterneededtoachieveadequatefluorescencesignaltonoiselevels.
- Afterlabeling,thecellsarewashedwithHHBSorfreshmediumbeforestartingtheexperiment.
| References&Citations |  CitationExplorer |
3DprintedlatticesasanactivationandexpansionplatformforTcelltherapy
Authors:BahmanDelalat,FrancesHarding,BatjargalGundsambuu,ElenaMDe-Juan-Pardo,FelixMWunner,Marie-LuiseWille,MarekJasieniak,KristenALMalatesta,HansJGriesser,AntonioSimula
Journal:Biomaterials(2017)
GD2-specificCARTCellsUndergoPotentActivationandDeletionFollowingAntigenEncounterbutcanbeProtectedFromActivation-inducedCellDeathbyPD-1Blockade
Authors:TessaGargett,WenboYu,GianpietroDotti,EricSYvon,SusanNChristo,JohnDHayball,IanDLewis,MalcolmKBrenner,MichaelPBrown
Journal:MolecularTherapy(2016)
InflammasomecomponentsASCandAIM2modulatetheacutephaseofbiomaterialimplant-inducedforeignbodyresponses
Authors:SusanNChristo,KerrilynRDiener,JimManavis,MicheleAGrimbaldeston,AkashBachhuka,KrasimirVasilev,JohnDHayball
Journal:Scientificreports(2016)
Hybridcore/shellmicroparticlesandtheiruseforunderstandingBIOLOGicalprocesses
Authors:AkashBachhuka,SusanNChristo,AlexCavallaro,KerrilynRDiener,AgnieszkaMierczynska,LouiseESmith,RomeoMarian,JimManavis,JohnDHayball,KrasimirVasilev
Journal:Journalofcolloidandinterfacescience(2015):9--17
ScrutinizingcalciumfluxoscillationsinTlymphocytestodeducethestrengthofstimulus
Authors:SusanNChristo,KerrilynRDiener,RobertENordon,MichaelPBrown,HansJGriesser,KrasimirVasilev,FaridCChristo,JohnDHayball
Journal:Scientificreports(2015):7760
IndividualandpopulationquantitativeanalysesofcalciumfluxinT-cellsactivatedonfunctionalizedmaterialsurfaces
Authors:SusanNChristo,GhafarTSarvestani,StefaniSGriesser,BryanRCoad,HansJGriesser,KrasimirVasilev,MichaelPBrown,KerrilynRDiener,JohnDHayball
Journal:AustralianJournalofChemistry(2012):45--49
Solid-statecaptureandreal-timeanalysisofindividualTcellactivationviaself-assemblyofbindingmultimericproteinsonfunctionalizedmaterialssurfaces
Authors:KerrilynRDiener,SusanNChristo,StefaniSGriesser,GhafarTSarvestani,KrasimirVasilev,HansJGriesser,JohnDHayball
Journal:Actabiomaterialia(2012):99--107
